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Estimating ruminal crude protein degradation from beef cattle feedstuff.

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  • 1State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, 100193, China.

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|August 8, 2019
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This study determined ruminal crude protein degradation in 12 Chinese feedstuffs using in vitro and in vivo methods. The in vitro gas production technique accurately estimates protein degradability, aiding in feedstuff evaluation.

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Area of Science:

  • Animal Nutrition
  • Ruminant Physiology
  • Feed Science

Background:

  • Accurate estimation of ruminal crude protein degradation is crucial for optimizing ruminant diets.
  • Traditional in vivo methods for assessing protein utilization are resource-intensive.
  • Development of reliable in vitro methods can provide a more efficient alternative for feedstuff evaluation.

Purpose of the Study:

  • To estimate the ruminal crude protein degradation of twelve common feedstuffs in China.
  • To validate the in vitro gas production method for determining protein degradability.
  • To establish regression equations correlating in vitro protein degradability (IVPD) with in vivo net protein utilization (NPU).

Main Methods:

  • In vivo determination of net protein utilization (NPU) for twelve feedstuffs.
  • In vitro incubation of feedstuffs to measure gas production and ammonia-nitrogen (NH3-N) incorporation into microbial proteins.
  • Linear regression analysis to establish relationships between gas production, NH3-N, IVPD, and NPU.

Main Results:

  • Significant linear regressions were observed between NH3-N and gas production at 6, 12, and 24 hours (r=0.9948, P<0.001; r=0.9874, P<0.01; r=0.9912, P<0.01).
  • Regression equations were generated to estimate in vitro protein degradability (IVPD) and its relationship with NPU for energy, protein, and roughage feed groups.
  • The in vitro gas production method demonstrated high accuracy in predicting protein degradation across different feed categories.

Conclusions:

  • The in vitro gas production method is a reliable and efficient tool for estimating ruminal protein degradation in feedstuffs.
  • The established regression equations can be used to predict in vivo protein utilization based on in vitro measurements.
  • This research provides valuable data for optimizing ruminant nutrition strategies in China through accurate feedstuff evaluation.