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Updated: Jan 21, 2026

Monitoring of Nanodrug Accumulation in Murine Breast Cancer Metastases
Published on: August 23, 2024
Detection of breast cancer stem cell gene mutations in circulating free DNA during the evolution of metastases
Zhe-Bin Liu1,2,3, Nader E Ezzedine4, Agda K Eterovic4
1Department of Breast Surgery, Fudan University Shanghai Cancer Center, Shanghai, 20032, China.
Purpose:
Limited knowledge exists on the detection of breast cancer stem cell (BCSC)-related mutations in circulating free DNA (cfDNA) from patients with advanced cancers. Identification of new cancer biomarkers may allow for earlier detection of disease progression and treatment strategy modifications.
Methods:
We conducted a prospective study to determine the feasibility and prognostic utility of droplet digital polymerase chain reaction (ddPCR)-based BCSC gene mutation analysis of cfDNA in patients with breast cancer.
Results:
Detection of quantitative BCSC gene mutation in cfDNA by ddPCR mirrors disease progression and thus may represent a valuable and cost-effective measure of tumor burden. We have previously shown that hematological and neurological expressed 1-like (HN1L), ribosomal protein L39 (RPL39), and myeloid leukemia factor 2 (MLF2) are novel targets for BCSC self-renewal, and targeting these genetic alterations could be useful for personalized genomic-based therapy.
Conclusion:
BCSC mutation detection in cfDNA may have important implications for diagnosis, prognosis, and serial monitoring.
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