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Mir-451 inhibits proliferation and migration of non-small cell lung cancer cells via targeting LKB1/AMPK

Y Liu1, H Li, L-H Li

  • 1Department of Respiratory Medicine, General Hospital of the North China Petroleum Administration, Renqiu, China. 15303175650@163.com.

European Review for Medical and Pharmacological Sciences
|August 8, 2019
PubMed
Abstract

Insights

MicroRNA-451 (miR-451) influences non-small cell lung cancer (NSCLC) cell proliferation and migration. miR-451 negatively correlates with liver kinase B1 (LKB1)/adenosine monophosphate-activated protein kinase (AMPK) signaling, suggesting a therapeutic target.

Area of Science:

  • Molecular Biology
  • Oncology
  • Biochemistry

Background:

  • Non-small cell lung cancer (NSCLC) remains a leading cause of cancer mortality.
  • MicroRNAs (miRNAs) play critical roles in cancer development and progression.
  • Dysregulation of specific miRNAs, such as miR-451, is implicated in NSCLC pathogenesis.

Purpose of the Study:

  • To investigate the role of miR-451 in NSCLC cell proliferation and apoptosis.
  • To elucidate the regulatory mechanism of miR-451 on liver kinase B1 (LKB1) and adenosine monophosphate-activated protein kinase (AMPK) signaling pathways.
  • To explore the potential of targeting miR-451 for NSCLC treatment.

Main Methods:

  • Utilized A549 NSCLC cells cultured under different conditions (control, miR-451 mimic, miR-451 inhibitor).
  • Assessed cell proliferation and migration using cell counting kit-8 (CCK8) and Transwell assays.
  • Quantified the expression levels of LKB1 and AMPK via Reverse Transcription-Polymerase Chain Reaction (RT-PCR).

Main Results:

  • miR-451 mimic significantly decreased cell migration, while miR-451 inhibitor increased it (p < 0.05).
  • Cell proliferation was altered by miR-451 modulation, with significant differences observed among groups (p < 0.05).
  • miR-451 mimic led to decreased expression of LKB1 and AMPK, whereas miR-451 inhibitor showed no significant difference compared to controls (p > 0.05).

Conclusions:

  • miR-451 negatively correlates with LKB1/AMPK signaling in NSCLC.
  • The LKB1/AMPK pathway is involved in NSCLC cell metabolism.
  • miR-451 may exert its tumor-suppressive effects in NSCLC by regulating the LKB1/AMPK pathway.

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