Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

27.3K
Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
27.3K
Induced Pluripotent Stem Cells01:06

Induced Pluripotent Stem Cells

5.4K
Stem cells are undifferentiated cells that divide and produce different cell types. Ordinarily, cells that have differentiated into a specific cell type are terminally differentiated; however, scientists have found a way to reprogram these mature cells so that they dedifferentiate and return to an unspecialized, proliferative state. These cells are pluripotent like embryonic stem cells—able to produce all cell types—and are called induced pluripotent stem cells (iPSCs).
Somatic...
5.4K
Overview of Fatty Acid Metabolism01:28

Overview of Fatty Acid Metabolism

36.7K
Lipids also are sources of energy that power cellular processes. Like carbohydrates, lipids are composed of carbon, hydrogen, and oxygen, but these atoms are arranged differently. Most lipids are nonpolar and hydrophobic. Major types include fats and oils, waxes, phospholipids, and steroids.
Fatty acids are catabolized in a process called beta-oxidation, which takes place in the matrix of the mitochondria and converts their fatty acid chains into two-carbon units of acetyl groups. The acetyl...
36.7K
Embryonic Stem Cells00:58

Embryonic Stem Cells

32.1K
Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
32.1K
What is Metabolism?00:52

What is Metabolism?

131.2K
Overview
131.2K
Adult Stem Cells01:33

Adult Stem Cells

33.4K
Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
33.4K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

PNPLA3 I148M Variant Activates Hepatic Stellate Cells via AMIGO2 Upregulation Using iPSC-Derived Model.

Liver international : official journal of the International Association for the Study of the Liver·2026
Same author

Recent progresses in cancer multidrug resistance and therapeutic options associated with protein damage response.

Protein & cell·2026
Same author

DGAT: a dual-graph attention network for inferring spatial protein landscapes from transcriptomics.

Nature communications·2026
Same author

Liver Disease Reveals KIF12 as a Critical Regulator of Mitochondria, Lysosome and Cilia Localization.

bioRxiv : the preprint server for biology·2026
Same author

THEMIS attenuates MASH by suppressing disease-associated hepatocyte induction and hepatocyte senescence in mice.

The Journal of clinical investigation·2026
Same author

Low dose radiation induces new protein synthesis that promotes cancer radiotherapy resistance.

Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy·2026

Related Experiment Video

Updated: Jan 21, 2026

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
07:37

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Published on: June 11, 2019

9.1K

Generation of Human Fatty Livers Using Custom-Engineered Induced Pluripotent Stem Cells with Modifiable SIRT1

Alexandra Collin de l'Hortet1, Kazuki Takeishi2, Jorge Guzman-Lepe1

  • 1Department of Pathology, University of Pittsburgh, Pittsburgh, PA, USA.

Cell Metabolism
|August 8, 2019
PubMed
Summary

Researchers developed a novel method to study human fatty liver disease using genetically engineered stem cells. This breakthrough allows for better understanding of liver disease progression and potential new treatments.

Keywords:
NAFLDNASHSIRT1cellular engineeringhepatic differentiationhuman fatty liverhuman iPSCsliver metabolism

More Related Videos

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
10:24

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Published on: October 5, 2011

22.0K
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

24.0K

Related Experiment Videos

Last Updated: Jan 21, 2026

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells
07:37

Efficient Differentiation of Human Pluripotent Stem Cells into Liver Cells

Published on: June 11, 2019

9.1K
Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells
10:24

Transfecting and Nucleofecting Human Induced Pluripotent Stem Cells

Published on: October 5, 2011

22.0K
Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
09:43

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus

Published on: April 23, 2014

24.0K

Area of Science:

  • Hepatology and Stem Cell Biology
  • Molecular Mechanisms of Liver Disease
  • Biofabrication and Tissue Engineering

Background:

  • The progression of fatty liver disease (steatosis) to non-alcoholic steatohepatitis and end-stage liver disease is not fully understood.
  • Sirtuin 1 (SIRT1) is implicated in metabolic regulation within hepatocytes and fatty liver development in animal models.
  • Studying human liver disease is challenging due to genetic variability, limiting in vivo research.

Purpose of the Study:

  • To investigate the role of SIRT1 in human fatty liver disease using patient-derived induced pluripotent stem cells (iPSCs).
  • To develop a biofabricated human liver tissue model for studying liver disease mechanisms.
  • To explore the potential of genetically engineered human liver tissue in disease research.

Main Methods:

  • Generation of human iPSCs with inducible SIRT1 expression control.
  • Differentiation of edited iPSCs into hepatocytes and subsequent SIRT1 knockdown.
  • Repopulation of decellularized rat livers with a mixture of human cells, including SIRT1-knockdown iPSC-derived hepatocytes.
  • Analysis of lipid accumulation, inflammatory markers, and metabolic profiles in the biofabricated liver tissue.

Main Results:

  • SIRT1 knockdown in iPSC-derived hepatocytes led to increased fatty acid biosynthesis and exacerbated fat accumulation.
  • The biofabricated human liver tissue exhibited macrosteatosis, a proinflammatory phenotype, and lipid/metabolic profiles similar to human fatty livers.
  • Genetically engineered human liver tissue successfully modeled key aspects of human fatty liver disease.

Conclusions:

  • Controllable SIRT1 expression in human iPSC-derived hepatocytes is crucial for regulating lipid metabolism.
  • Biofabricated, genetically edited human liver tissue serves as a viable model for studying human fatty liver disease.
  • This approach offers a powerful new tool for investigating liver biology and disease mechanisms in humans.