Fluctuation Scaling of Neuronal Firing and Bursting in Spontaneously Active Brain Circuits

We employed high-density microelectrode arrays to investigate spontaneous firing patterns of neurons in brain circuits of the primary somatosensory cortex (S1) in mice. We recorded from over 150 neurons for 10$$min in each of eight different experiments, identified their location in S1, sorted their action potentials (spikes), and computed their power spectra and inter-spike interval (ISI) statistics. Of all persistently active neurons, 92% fired with a single dominant frequency - regularly firing neurons (RNs) - from 1 to 8$$Hz while 8% fired in burst with two dominant frequencies - bursting neurons (BNs) - corresponding to the inter-burst (2-6$$Hz) and intra-burst intervals (20-160$$Hz). RNs were predominantly located in layers 2/3 and 5/6 while BNs localized to layers 4 and 5. Across neurons, the standard deviation of ISI was a power law of its mean, a property known as fluctuation scaling, with a power law exponent of 1 for RNs and 1.25 for BNs. The power law implies that firing and bursting patterns are scale invariant: the firing pattern of a given RN or BN resembles that of another RN or BN, respectively, after a time contraction or dilation. An explanation for this scale invariance is discussed in the context of previous computational studies as well as its potential role in information processing.