Related Experiment Videos
Restriction of anti-peptide antibody specificity by enzyme-modified Sepharose-peptide immunoadsorbents
M L Trinca1, E Muratti, M Camera
1Istituto Regina Elena for Cancer Research, Lab. of Biochemistry, Rome, Italy.
Biochimie
|June 1, 1988
Abstract:
Sepharose-peptide immunoadsorbents, employed for the isolation of specific antibodies from the sera of rabbits immunized with carrier protein-peptide conjugates, were digested with suitable proteolytic enzymes, in order to obtain the splitting of a part of the peptide bound to the gel. This new modified immunoadsorbent can be advantageously used for the isolation of antibody subsets, that do not cross-react with related peptides exhibiting high sequence homology with the immunogens.