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Updated: Jan 21, 2026
06:55
Protein Isolation from the Developing Embryonic Mouse Heart Valve Region
Published on: September 23, 2014
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SNARE protein SEC22B regulates early embryonic development
Shin-Rong J Wu1,2, Rami Khoriaty3, Stephanie H Kim1,2
1Program in Immunology, University of Michigan Medical School, Ann Arbor, USA.
Scientific Reports
|August 9, 2019
Summary
Sec22b protein is essential for early embryogenesis. Its deletion in hematopoietic/endothelial cells causes embryonic death, but deletion in CD11c cells allows survival, revealing tissue-specific roles.
Area of Science:
- Cell Biology
- Developmental Biology
- Genetics
Background:
- The SNARE protein SEC22B is crucial for cellular processes like phagocytosis and secretion.
- Previous studies on SEC22B's functions were limited to in vitro experiments.
Purpose of the Study:
- To investigate the in vivo role of Sec22b during early embryogenesis.
- To determine tissue-specific functions of Sec22b using conditional knockout mice.
Main Methods:
- Utilized Cre-Lox mouse models for tissue-specific deletion of Sec22b.
- Examined developmental outcomes following germline and conditional Sec22b deletions.
Main Results:
- Germline deletion of Sec22b led to embryonic lethality at E8.5.
- Deletion in hematopoietic/endothelial cells also caused in utero death.
- Mice with Sec22b deleted in CD11c+ cells survived to adulthood, indicating partial rescue.
Conclusions:
- Sec22b plays a vital role in early embryogenesis.
- Its function is critical in both hematopoietic/endothelial tissues and other undefined compartments.
- Conditional deletion highlights tissue-specific requirements for Sec22b during development.
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