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Updated: Jan 21, 2026

Protein Isolation from the Developing Embryonic Mouse Heart Valve Region
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SNARE protein SEC22B regulates early embryonic development.

Shin-Rong J Wu1,2, Rami Khoriaty3, Stephanie H Kim1,2

  • 1Program in Immunology, University of Michigan Medical School, Ann Arbor, USA.

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|August 9, 2019
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Summary

Sec22b protein is essential for early embryogenesis. Its deletion in hematopoietic/endothelial cells causes embryonic death, but deletion in CD11c cells allows survival, revealing tissue-specific roles.

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Area of Science:

  • Cell Biology
  • Developmental Biology
  • Genetics

Background:

  • The SNARE protein SEC22B is crucial for cellular processes like phagocytosis and secretion.
  • Previous studies on SEC22B's functions were limited to in vitro experiments.

Purpose of the Study:

  • To investigate the in vivo role of Sec22b during early embryogenesis.
  • To determine tissue-specific functions of Sec22b using conditional knockout mice.

Main Methods:

  • Utilized Cre-Lox mouse models for tissue-specific deletion of Sec22b.
  • Examined developmental outcomes following germline and conditional Sec22b deletions.

Main Results:

  • Germline deletion of Sec22b led to embryonic lethality at E8.5.
  • Deletion in hematopoietic/endothelial cells also caused in utero death.
  • Mice with Sec22b deleted in CD11c+ cells survived to adulthood, indicating partial rescue.

Conclusions:

  • Sec22b plays a vital role in early embryogenesis.
  • Its function is critical in both hematopoietic/endothelial tissues and other undefined compartments.
  • Conditional deletion highlights tissue-specific requirements for Sec22b during development.