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Updated: Jan 21, 2026

Conventional BODIPY Conjugates for Live-Cell Super-Resolution Microscopy and Single-Molecule Tracking
Published on: June 8, 2020
Photophysical properties and application in live cell imaging of B,B-fluoro-perfluoroalkyl BODIPYs
Atsuhiko Taniguchi1, Taka Sawazaki1, Yusuke Shimizu1
1Graduate School of Pharmaceutical Sciences , The University of Tokyo , 7-3-1, Hongo, Bunkyo-ku , Tokyo 113-0033 , Japan . Email: ysohma@mol.f.u-tokyo.ac.jp ;
Abstract:
The photophysical properties of newly identified B,B-fluoro-perfluoroalkyl BODIPYs (2 and 3), which possess a fluoro group and a trifluoromethyl or pentafluoroethyl group at the boron center, were investigated. B,B-Fluoro-perfluoroalkyl BODIPYs 2 and 3 exhibited better photophysical/chemical properties than B,B-difluoro-BODIPY 1, as follows: (1) higher photostability both in methanol solvent and in a live cell environment, (2) higher stability against acid degradation, and (3) improved fluorescence signal-to-noise ratios in a cell system. These favorable properties of B,B-fluoro-perfluoroalkyl BODIPYs are likely due to the highly electron-withdrawing nature of the perfluoroalkyl groups on the boron atom, which reduces the reactivity to 1O2 and strengthens the complexation of the dipyrromethene ligands to the boron atom. Thus, B,B-fluoro perfluoroalkyl BODIPYs may be useful functional molecules for various applications.
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