Measuring Microglial Turnover in the Adult Brain
Diego Gomez-Nicola1, Gemma L Fryatt2, Katharine E Askew2
1School of Biological Sciences, University of Southampton, Southampton, UK. d.gomez-nicola@soton.ac.uk.
Abstract:
Microglia are the main resident immunocompetent cells of the brain with key roles in brain development, homeostasis, and function. Recent reports have started to shed light on the homeostatic mechanisms regulating the composition and turnover of the microglial population under physiological conditions from development to ageing, but our knowledge of the dynamics of microglia is incomplete. Therefore, it appears relevant to provide a standardized approach to quantify the turnover of microglia, with direct application to create a greater understanding of the dynamics of this cell population, and how it may contribute to the pathogenesis and/or progression of neurological disorders. Here we describe a robust immunohistochemical method to analyze microglial proliferation in mouse brain, aiming at providing a shared and universal approach to analyze microglial dynamics across different laboratories.
Related Concept Videos
Turnover Number and Catalytic Efficiency
Chymotrypsin is a pancreatic enzyme that breaks down proteins during digestion....
Adult Stem Cells
Cardiopulmonary Resuscitation I: Adult
Relationship with Other Adult Family Members and Siblings
Brain Waves
Measures of Central Tendency


