Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Deconvolution01:20

Deconvolution

555
Deconvolution, also known as inverse filtering, is the process of extracting the impulse response from known input and output signals. This technique is vital in scenarios where the system's characteristics are unknown, and they must be inferred from the observable signals.
Deconvolution involves several mathematical techniques to derive the impulse response. One common approach is polynomial division. In this method, the input and output sequences are treated as coefficients of...
555
Kinetics of Drug Elimination01:17

Kinetics of Drug Elimination

4.1K
Eliminating drugs from the body is a vital process that occurs through excretion or metabolism. Understanding the kinetics of drug elimination is crucial for drug development, dosage determination, and optimizing patient outcomes.
Drug clearance depends on the rate of drug elimination and its plasma concentration. Another important parameter is the half-life of a drug, which is the time required for its concentration to decrease by half. In most cases, drug clearance follows first-order...
4.1K
Enhanced Elimination of Poison01:26

Enhanced Elimination of Poison

876
Poison can be effectively removed from the gastrointestinal (GI) tract through various decontamination procedures.
Antidotes serve a crucial role in counteracting the effects of poison by inhibiting enzymes responsible for producing harmful drug metabolites. In some cases, these toxic metabolites can be neutralized by endogenous cosubstrates, which are maintained at specific concentrations to prevent interaction with cellular macromolecules and subsequent cell death.
Renal excretion is the...
876
Radical Formation: Elimination00:51

Radical Formation: Elimination

2.2K
Another method of radical formation is the elimination process. It is the opposite of the addition route and is driven by the instability of the radical. For example, as depicted in Figure 1, dibenzoyl peroxide yields a pair of unstable radicals upon homolysis. Given its instability, this radical spontaneously undergoes elimination via a C–C bond cleavage to form a relatively more stable phenyl radical. The mechanism involves cleavage of the bond between the α and β positions with respect...
2.2K
Elimination Reactions02:25

Elimination Reactions

16.6K
A nucleophile can react with an alkyl halide to give the substitution product by displacing the halogen. Or it can function as a base to give the elimination product by deprotonation of the neighboring carbon to form an alkene. In an elimination reaction, the substrate loses two groups from adjacent carbons forming at least one π bond. The carbon attached to the halogen is called the α carbon, while the adjacent carbon is called the β carbon; hence, these reactions are called...
16.6K
Elimination Kinetics: First-Order and Zero-Order01:05

Elimination Kinetics: First-Order and Zero-Order

2.7K
Eliminating drugs from the body is a vital process that occurs through excretion or metabolism. Understanding the kinetics of drug elimination is crucial for drug development, dosage determination, and optimizing patient outcomes.
Drug clearance depends on the rate of drug elimination and its plasma concentration. Another important parameter is a drug's half-life, which is the time required for its concentration to decrease by half. In most cases, drug clearance follows first-order...
2.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Revealing the Fate of Isomeric Monounsaturated Fatty Acids in <i>Enterococcus faecalis</i> Membrane Lipids and Their Influence on Antimicrobial Susceptibility.

ACS infectious diseases·2026
Same author

Design of Fluorescent Membrane Scaffold Proteins for Nanodiscs.

bioRxiv : the preprint server for biology·2026
Same author

LetA defines a structurally distinct transporter family.

Nature·2026
Same author

18-Crown-6-ether Utilizes Distinct Allosteric Interactions to Uncouple Transport by the Multidrug Efflux Pump EmrE.

Biochemistry·2025
Same author

Author Correction: A universal language for finding mass spectrometry data patterns.

Nature methods·2025
Same author

A Fast Neural Network for Isotopic Charge State Assignment.

Journal of the American Chemical Society·2025
Same journal

Characterization of Alkane Oxidation Products in a Corona-Discharge Reactor Using Ammonia-Doped Ion Mobility-Mass Spectrometry.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Integration of a Modified Synchrotron Radiation Photoionization Time-of-Flight Mass Spectrometer with a Residual Gas Analyzer for Complementary Detection of Catalytic Products with Different Ionization Energies.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Screen for Tissue-Specific Markers of Drug-Induced Phospholipidosis Using Mass Spectrometry Imaging.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Molecular Framework Modification in Mass Spectrometry: Atom Exchange, Insertion, and Deletion.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Interlaboratory Comparison of Orbitrap Analyzers in OrbiSIMS: Noise Parameters, Intensity Scaling, and Detection Limits.

Journal of the American Society for Mass Spectrometry·2026
Same journal

Isotope-Coded Derivatization Enables Accurate LC-MS/MS Quantification of Urinary THC-COOH without Isotope-Labeled Cannabinoid Standards.

Journal of the American Society for Mass Spectrometry·2026
See all related articles

Related Experiment Video

Updated: Jan 21, 2026

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
06:42

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

Published on: April 6, 2017

12.0K

Eliminating Artifacts in Electrospray Deconvolution with a SoftMax Function.

Michael T Marty1

  • 1Department of Chemistry and Biochemistry, University of Arizona, 1306 E University Blvd, Tucson, AZ, 85721, USA. mtmarty@email.arizona.edu.

Journal of the American Society for Mass Spectrometry
|August 9, 2019
PubMed
Summary
This summary is machine-generated.

The UniDec algorithm for mass spectrometry deconvolution can create artifacts. Applying a SoftMax function to charge state distributions significantly reduces these artifacts, improving data accuracy even with complex datasets.

Keywords:
DeconvolutionElectrosprayIntact massMass spectrometryNative MS

More Related Videos

Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.8K
Equipment Setup and Artifact Removal for Simultaneous Electroencephalogram and Functional Magnetic Resonance Imaging for Clinical Review in Epilepsy
10:23

Equipment Setup and Artifact Removal for Simultaneous Electroencephalogram and Functional Magnetic Resonance Imaging for Clinical Review in Epilepsy

Published on: June 23, 2023

2.7K

Related Experiment Videos

Last Updated: Jan 21, 2026

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen
06:42

A Multi-hole Cryovial Eliminates Freezing Artifacts when Muscle Tissues are Directly Immersed in Liquid Nitrogen

Published on: April 6, 2017

12.0K
Analysis of SEC-SAXS data via EFA deconvolution and Scatter
10:59

Analysis of SEC-SAXS data via EFA deconvolution and Scatter

Published on: January 28, 2021

9.8K
Equipment Setup and Artifact Removal for Simultaneous Electroencephalogram and Functional Magnetic Resonance Imaging for Clinical Review in Epilepsy
10:23

Equipment Setup and Artifact Removal for Simultaneous Electroencephalogram and Functional Magnetic Resonance Imaging for Clinical Review in Epilepsy

Published on: June 23, 2023

2.7K

Area of Science:

  • Mass spectrometry
  • Proteomics
  • Biophysical chemistry

Background:

  • Electrospray mass spectrometry is crucial for analyzing biomolecules.
  • UniDec offers rapid deconvolution of mass spectra into mass and charge states.
  • UniDec can generate artifacts, especially with complex or noisy data.

Purpose of the Study:

  • To improve the accuracy of UniDec by reducing deconvolution artifacts.
  • To enhance the robustness of mass spectrometry data analysis.
  • To refine charge state assignment in complex mass spectra.

Main Methods:

  • Implementation of a SoftMax function applied to charge state distributions.
  • Testing the modified algorithm on diverse mass spectrometry datasets.
  • Evaluation of artifact reduction and accuracy improvement.

Main Results:

  • The SoftMax function effectively assigns a primary charge state to each data point.
  • Significant reduction in deconvolution artifacts observed across multiple datasets.
  • Improved performance demonstrated even with overlapping charge states.

Conclusions:

  • The SoftMax modification enhances UniDec's reliability for mass spectrometry data.
  • This approach offers a robust solution for minimizing deconvolution artifacts.
  • The refined method improves the accuracy of mass and charge state determination.