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Updated: Jan 21, 2026
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Design and Development of Aptamer–Gold Nanoparticle Based Colorimetric Assays for In-the-field Applications
Published on: June 23, 2016
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Electrochromic, Closed-Bipolar Electrodes Employing Aptamer-Based Recognition for Direct Colorimetric Sensing
Analytical Chemistry
|August 9, 2019
Summary
Researchers developed a new colorimetric aptamer-based sensor using a closed-bipolar electrode (C-BPE) system. This novel sensor enables quantitative detection of target molecules like ATP and tobramycin through a visible color change, offering a user-friendly alternative for point-of-use applications.
Area of Science:
- Electrochemistry
- Biosensors
- Analytical Chemistry
Background:
- Electrochemical aptamer-based (E-AB) sensors offer sensitive detection but often require specialized equipment.
- Developing simpler, visually interpretable detection methods is crucial for broader accessibility.
- Colorimetric assays provide a qualitative or semi-quantitative readout without complex instrumentation.
Purpose of the Study:
- To adapt the E-AB sensor platform for colorimetric detection using a closed-bipolar electrode (C-BPE) system.
- To develop a quantitative method for target molecule detection based on the rate of color change.
- To demonstrate the potential for naked-eye and smartphone-based analysis for point-of-use applications.
Main Methods:
- Modification of a C-BPE system with a redox-labeled aptamer specific for target molecules (adenosine triphosphate and tobramycin).
- Utilizing an electrochromic Prussian white (PW) thin-film indicator electrode that changes color to Prussian blue (PB) upon reduction.
- Employing a potassium ferricyanide-catalyzed reaction to facilitate the color change rate, correlating it with analyte concentration.
Main Results:
- Demonstrated quantitative detection of target analytes based on the rate of color change from PW to PB.
- Established a clear relationship between analyte concentration and the speed of the observed color transition.
- Validated the system using well-characterized aptamers for adenosine triphosphate (ATP) and tobramycin.
Conclusions:
- The developed C-BPE E-AB sensor provides a user-friendly, colorimetric method for quantitative analyte detection.
- This platform eliminates the need for potentiostats and voltammetric analysis, simplifying E-AB sensing.
- The sensor shows promise for point-of-use applications, especially when coupled with smartphone-based colorimetric analysis.
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