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Updated: Jan 21, 2026

Synthesis of Core-shell Lanthanide-doped Upconversion Nanocrystals for Cellular Applications
Published on: November 10, 2017
Regulating Fluorescent Aptamer-Sensing Behavior of Zeolitic Imidazolate Framework (ZIF-8) Platform via Lanthanide Ion
Ya-Bo Hao1,2, Zhen-Shu Shao1, Chen Cheng1
1Department of Pharmaceutical Analysis , China Pharmaceutical University , Nanjing 210009 , China.
Abstract:
Nanoscale metal-organic frameworks (NMOFs) have been proved to be effective quenching platforms for fluorescent detection of DNA via fluorophore-quencher pairs. Zeolitic imidazolate framework-8 (ZIF-8) is one type of the most promising NMOFs because of its excellent biocompatibility and easy preparation. However, ZIF-8 is rarely used as platforms for fluorescence sensing of DNA because of its bad fluorescence quenching property. In this study, lanthanide ions were doped into ZIF-8 to regulate its fluorescence quenching behavior. The La3+ doped ZIF-8 (ZIF-8-La) showed the best quenching efficiency on dye-labeled DNA. The signal-to-background ratio was around 3 times higher than ZIF-8. Furthermore, a core-shell La3+-doped ZIF-8 (CS-ZIF-8-La) was designed to modify more La3+ on the surface of ZIF-8. Compared with ZIF-8-La, the CS-ZIF-8-La exhibited the same fluorescence sensing behavior toward positive-dye-labeled DNA, but showed completely contrary quenching property on the negative-dye-labeled DNA. On the basis of this phenomenon, CS-ZIF-8-La was successfully used as quenching platform for designing a ratiometric sensor for DNA and microRNA.
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