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Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging
Published on: July 9, 2008
The immunoregulatory effect of sulfated Echinacea purpurea polysaccharide on chicken bone marrow-derived dendritic
Lili Yao1, Lin Bai1, Ying Tan1
1College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, PR China.
Abstract:
Echinacea purpurea polysaccharide (EPP) was obtained by modern separation technology and sulfated EPP (sEPP) was prepared by sulfation modification. The immunological effects of EPP and sEPP were compared on chicken bone marrow-derived dendritic cells (chBM-DCs). The results showed that the surface marker expression of CD11c and CD80 was increased after chBM-DCs were cultured with three dosage of sEPP, especially in sEPPM group. Three dosage of sEPP, EPPL and LPS could significantly enhance the effects of chBM-DCs on the proliferation of allogenic mixed lymphocytes. After chBM-DCs treatment with EPP or sEPP in vitro, the levels of IL-2 of sEPPH and EPPM groups were significantly higher than those of LPS group (P < 0.05). All sEPP and EPP groups could enhance the level of IFN-γ and down-regulated the level of IL-4 and IL-10. Results indicated that both sEPP and EPP had immunoregulatory effects on chBM-DCs, sEPP possessed better immunoregulatory effects as compared with EPP.
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