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Atomic Force Microscopy Imaging and Force Spectroscopy of Supported Lipid Bilayers
Published on: July 22, 2015
Atomic Force Microscopy as a Tool in Nanobiology Part II: Force Spectroscopy in Genomics and Proteomics
Lilian T Costa1, Stefan Thalhammer1, Wolfgang M Heckl1
1Department for Geo-and Environmental Sciences, GeobioCenter and Center for Nanoscience, Ludwig-Maximilians-University, Theresienstr. 41, 80333 Munich, Germany.
Abstract:
We present possible applications of Atomic Force Microscopy (AFM) as a force spectroscopy tool in genomics and proteomics. AFM applications in these fields have opened new opportunities for studying the mechanical properties of biomolecules and their interactions in their native environment, as well as in determining the binding affinity of DNA proteins in dependence with the target DNA sequence for further correlative studies on physical affinity and biological relevance of the controlled gene. Furthermore, force spectroscopy is a powerful analytical tool to investigate structural and functional features of biomolecules. Altogether, these tools have revealed useful application in genomics and proteomics.
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