Related Experiment Video

Updated: Jan 21, 2026

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes
10:00

High-throughput Quantitative Real-time RT-PCR Assay for Determining Expression Profiles of Types I and III Interferon Subtypes

Published on: March 24, 2015

13.8K

Correlation Between Interferon Alpha Receptor Protein Expression and Sensitivity to Interferon Alpha Subtypes in

Toshio Ariyasu1, Noboru Fujioka1, Shigeto Yamamoto1

  • 1Fujisaki Institute, Hayashibara Biochemical Laboratories, Inc., 675-1 Fujisaki, Okayama 702-8006.

Cancer Genomics & Proteomics
|August 10, 2019
PubMed
Abstract

Insights

Interferon-alpha-8 (IFN-α8) shows greater renal cell carcinoma (RCC) growth inhibition than IFN-α2. This sensitivity is linked to the expression of the interferon-alpha receptor (IFNAR)-2(c) protein, suggesting its potential as a prognostic marker in RCC treatment.

Area of Science:

  • Oncology
  • Immunology
  • Molecular Biology

Background:

  • Previous studies characterized interferon-alpha (IFN-α) subtypes' antitumor and immunological effects on renal cell carcinoma (RCC).
  • The precise mechanisms driving differential biologic activities among IFN-α subtypes remain unclear.
  • Investigating cellular sensitivity to IFN-α subtypes requires understanding interferon-alpha receptor (IFNAR)-1 and IFNAR-2 subunit expression in RCC cell lines.

Purpose of the Study:

  • To compare the antiproliferative effects of natural IFN-α subtypes (IFN-α2 and IFN-α8) on RCC cell lines.
  • To determine the expression levels of IFNAR-1 and IFNAR-2 subunits in these cell lines.
  • To correlate IFN-α activity with IFNAR expression levels to elucidate differential cellular sensitivities.

Main Methods:

  • Antiproliferative effects of IFN-α2 and IFN-α8 were assessed on eight RCC cell lines.
  • Interferon-alpha receptor (IFNAR)-1 and IFNAR-2 expression were quantified using RT-PCR and Western blotting.
  • Statistical analysis examined the correlation between the 50% effective IFN dose (ED50) for growth inhibition and IFNAR expression levels.

Main Results:

  • IFN-α8 demonstrated more potent growth inhibition than IFN-α2 across most examined RCC cell lines.
  • The ED50 for IFN-α8 was below 1000 IU/ml in six cell lines, compared to three cell lines for IFN-α2.
  • IFN-α subtype sensitivity strongly correlated with the expression level of the long form of IFNAR-2, IFNAR-2(c), in seven of eight cell lines.

Conclusions:

  • IFN-α8 exhibits superior antiproliferative activity against RCC compared to IFN-α2.
  • IFNAR-2(c) protein expression levels are closely associated with cellular sensitivity to different IFN-α subtypes.
  • IFNAR-2(c) expression intensity may serve as a valuable prognostic marker for selecting specific IFN-α subtypes in RCC therapy.

More Related Videos

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α08:26

Development and Validation of an Ultrasensitive Single Molecule Array Digital Enzyme-linked Immunosorbent Assay for Human Interferon-α

Published on: June 14, 2018

12.5K
Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

6.8K

Related Concept Videos

G-protein Coupled Receptors01:21

G-protein Coupled Receptors

G-protein coupled receptors are ligand binding receptors that indirectly affect changes in the cell. The actual receptor is a single polypeptide that transverses the cell membrane seven times creating intracellular and extracellular loops. The extracellular loops create a ligand specific pocket which binds to neurotransmitters or hormones. The intracellular loops holds onto the G-protein.
131.6K
Adrenergic Receptors: ɑ Subtype01:31

Adrenergic Receptors: ɑ Subtype

Adrenoceptors are classified into α and ꞵ classes based on their potencies to catecholamine agonists. α-adrenoceptors show the following order of catecholamine potency:
Adrenaline ≥ Noradrenaline >> Isoprenaline
α-adrenoceptors are further divided into α1 and α2-adrenoceptors.
α1-Adrenoceptors: These receptors are located postsynaptically on the effector organs and cause constriction of smooth muscle mediated by activation of phospholipase...
2.8K
Adrenergic Receptors: β Subtype01:26

Adrenergic Receptors: β Subtype

β-adrenoceptors have varied sensitivities towards adrenaline, noradrenaline, and isoprenaline. The order of agonist potency is as follows:
Isoprenaline > Adrenaline > Noradrenaline
Neurotransmitter binding to these receptors causes activation of adenylyl cyclase resulting in increased concentrations of cAMP and modulation of calcium ion channels within the cell. They are further classified into β1, β2, and β3 subtypes.
β1-adrenoceptors: β1-adrenoceptors...
3.6K
Cell Lines01:16

Cell Lines

A cell line is a population of cells grown in vitro that can be subcultured over several generations. Normal cells cease to divide after a certain number of cell divisions, a process known as replicative senescence. This number, called the Hayflick limit, was conceptualized by Leonard Hayflick in 1961 when he observed that fetal cells grown in culture could only divide 40-60 times. This limit is due to the shortening of the telomeres during each round of cell division, preventing cell division...
10.0K
Conservation of Protein Domains Over Different Proteins02:26

Conservation of Protein Domains Over Different Proteins

Protein domains are small structurally independent units that are part of a single amino acid chain.  Although these domains are often structurally independent, they may rely on synergistic effects to perform their functions as part of a larger protein. Protein domains may be conserved within the same organism, as well as across different organisms.
A limited set of protein domains often duplicate and recombine during evolution. These domains can be organized in different combinations to...
14.1K
Correlations02:20

Correlations

Correlation means that there is a relationship between two or more variables (such as ice cream consumption and crime), but this relationship does not necessarily imply cause and effect. When two variables are correlated, it simply means that as one variable changes, so does the other. We can measure correlation by calculating a statistic known as a correlation coefficient. A correlation coefficient is a number from -1 to +1 that indicates the strength and direction of the relationship between...
35.8K