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Updated: Jan 21, 2026

VIGS-Mediated Forward Genetics Screening for Identification of Genes Involved in Nonhost Resistance
08:17

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Published on: August 23, 2013

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Expression Analysis for the Identification of Genes Involved in Acquired Resistance to Cisplatin in Osteosarcoma

Koutarou Takazawa1, Hiroyuki Tsuchiya1, Ueda Yoshimichi2

  • 1Department of Orthopaedic Surgery, Graduate School of Medical Science, Kanazawa University, 13-1 Takara-machi, Kanazawa.

Cancer Genomics & Proteomics
|August 10, 2019
PubMed
Abstract

Insights

This study identified early gene expression changes in cancer cells developing resistance to cisplatin. Understanding these changes can help improve chemotherapy effectiveness.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genomics

Background:

  • Tumor cells can develop tolerance to anticancer drugs with repeated administration.
  • Cisplatin resistance is a significant challenge in cancer chemotherapy.
  • Early identification of resistance mechanisms is crucial for effective treatment.

Purpose of the Study:

  • To analyze gene expression in cisplatin-resistant cells.
  • To identify early molecular changes during the development of cisplatin resistance.
  • To understand the genetic basis of acquired tolerance to cisplatin.

Main Methods:

  • Established cisplatin-resistant human osteosarcoma sublines (OST/R).
  • Utilized cDNA expression microarrays for gene expression analysis.
  • Investigated gene expression profiles following prolonged cisplatin exposure.

Main Results:

  • Identified 17 genes with increased and 14 genes with decreased expression in resistant cells.
  • Observed alterations in genes related to DNA repair, apoptosis, cell cycle, and proliferation.
  • Pinpointed specific genes exhibiting early changes in expression during resistance development.

Conclusions:

  • Early detection of altered gene expression can predict cisplatin resistance.
  • Understanding these early genetic changes may enhance cisplatin's therapeutic efficacy.
  • This research provides insights into molecular mechanisms of acquired chemoresistance.
Keywords:
DNA microarrayOsteosarcomacisplatindrug-resistance

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