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Proteomic Studies of Galectin-3 Expression in Human Thyroid Diseases by Immunodetection
Pantipa Subhasitanont1, Chantragan Srisomsap2, Phaibul Punyarit3
1Laboratory of Biochemistry, Chulabhorn Research Institute.
Cancer Genomics & Proteomics
|August 10, 2019
Summary
Galectin-3 protein expression is significantly elevated in thyroid papillary carcinoma and other malignant cancers. This finding suggests galectin-3 may serve as a biomarker for cancer detection.
Area of Science:
- Biochemistry
- Oncology
- Molecular Biology
Background:
- Galectin-3 is a protein implicated in various biological processes.
- Altered galectin-3 expression is observed in several diseases.
Purpose of the Study:
- To investigate galectin-3 expression levels in different thyroid diseases.
- To assess the potential of galectin-3 as a biomarker for thyroid papillary carcinoma.
Main Methods:
- One-dimensional (1-DE) and two-dimensional (2-DE) immunoblotting techniques were employed.
- Analysis focused on comparing galectin-3 expression in neoplastic and non-neoplastic thyroid tissues.
Main Results:
- Galectin-3 expression was markedly elevated in thyroid papillary carcinoma compared to benign thyroid conditions.
- Elevated galectin-3 was also detected in malignant cancers of bone, breast, colon, esophagus, larynx, lung, and ovary.
- Thyroid papillary carcinoma with metastasis showed multiple galectin-3 bands on 1-DE immunoblotting.
Conclusions:
- Galectin-3 is significantly overexpressed in thyroid papillary carcinoma.
- Galectin-3 may function as a valuable diagnostic biomarker for thyroid papillary carcinoma and other malignancies.
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