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Related Experiment Video

Updated: Jan 21, 2026

Dissection and Culture of Mouse Embryonic Kidney

Dissection and Culture of Mouse Embryonic Kidney

Published on: May 17, 2017

Metanephric organ culture.

Robin L Maser¹, Brenda S Magenheimer², James P Calvet²

¹Department of Clinical Laboratory Sciences, University of Kansas Medical Center, Kansas City, KS, United States; Department of Biochemistry and Molecular Biology, University of Kansas Medical Center, Kansas City, KS, United States; Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS, United States.

Methods in Cell Biology

|August 10, 2019

Summary

Metanephric organ culture, an ex vivo embryonic kidney model, aids in understanding kidney development and polycystic kidney disease. This method helps identify disease mechanisms and evaluate potential therapies for renal cyst initiation.

Keywords:

Cystic dilation Cystic index Embryonic kidney Ex vivo In vitro kidney culture Kidney culture Polycystic kidney disease Whole mount labeling Whole mount staining

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Last Updated: Jan 21, 2026

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Published on: May 17, 2017

Primary Culture and Plasmid Electroporation of the Murine Organ of Corti.

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Preparation of 2-dGuo-Treated Thymus Organ Cultures

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Published on: August 28, 2008

Area of Science:

Developmental Biology
Nephrology
Genetics

Background:

Metanephric organ culture, or ex vivo embryonic kidney culture, was established mid-20th century to study mammalian kidney development.
Early research utilized this model to investigate non-genetic factors in polycystic kidney disease (PKD) cyst initiation.

Purpose of the Study:

To provide detailed protocols for establishing, maintaining, and treating metanephric organ cultures.
To outline common secondary analyses for these cultures.
To highlight the resurgence and utility of this model in PKD research.

Main Methods:

Establishment and maintenance of metanephric organ cultures.
Treatment of cultures to study disease mechanisms.
Performance of secondary analyses on cultured kidney tissues.

Main Results:

Metanephric organ culture has provided insights into basic mechanisms of cystic dilation in the kidney.
The model has facilitated the identification of pathogenic pathways and potential therapeutic targets for PKD.
It serves as a system for evaluating therapeutic agents for renal diseases.

Conclusions:

Metanephric organ culture is a valuable tool for studying kidney development and diseases like PKD.
This ex vivo model is crucial for understanding cystogenesis and testing interventions.
Detailed protocols enhance the reproducibility and application of this technique in renal research.