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ADPKD cell proliferation and Cl--dependent fluid secretion

Gail A Reif1, Darren P Wallace1

  • 1Departments of Internal Medicine and Molecular and Integrative Physiology, and The Jared Grantham Kidney Institute, University of Kansas Medical Center, Kansas City, KS, United States.

Methods in Cell Biology
|August 10, 2019
PubMed

Insights

Autosomal dominant polycystic kidney disease (ADPKD) involves cyst growth driven by cell proliferation and fluid secretion. This study details methods to measure these processes for developing new ADPKD therapies.

Area of Science:

  • Nephrology
  • Genetics
  • Cell Biology

Background:

  • Autosomal dominant polycystic kidney disease (ADPKD) is a common genetic kidney disorder.
  • It causes cyst formation, inflammation, fibrosis, and kidney function decline.
  • The precise mechanisms of cystogenesis in ADPKD are not fully understood.

Purpose of the Study:

  • To explore cellular and molecular mechanisms of ADPKD cyst epithelial cell proliferation.
  • To investigate mechanisms of chloride-dependent fluid secretion in ADPKD.
  • To evaluate potential therapeutic strategies targeting cyst growth pathways.

Main Methods:

  • Utilizing in vitro assays to study ADPKD cyst epithelial cells.
  • Measuring ADPKD cell proliferation rates.
  • Quantifying transepithelial chloride secretion and net fluid transport.

Main Results:

  • Established methods for assessing key ADPKD cyst growth drivers.
  • Provided a framework for evaluating therapeutic interventions in controlled experimental settings.
  • Highlighted the roles of cell proliferation and fluid secretion in ADPKD pathogenesis.

Conclusions:

  • In vitro assays are crucial for understanding ADPKD mechanisms.
  • These methods facilitate the evaluation of novel therapeutic targets.
  • Further research using these assays can advance ADPKD treatment strategies.

Keywords:
Anion secretionAutosomal dominant polycystic kidney diseaseCyst growthIn vitro modelsIon transport

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