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Genome-Wide Transcript and Small RNA Profiling Reveals Transcriptomic Responses to Heat Stress
Juan He1,2, Zengming Jiang1, Lei Gao1
1Guangdong Provincial Key Laboratory for Plant Epigenetics, Longhua Bioindustry and Innovation Research Institute, College of Life Sciences and Oceanography, Shenzhen University, Shenzhen, Guangdong 518060, China.
Plant Physiology
|August 10, 2019
Summary
Climate change causes heat stress in crops. This study reveals molecular responses in maize, identifying key regulators for improving heat tolerance in plants.
Area of Science:
- Plant Biology
- Molecular Biology
- Genomics
Background:
- Climate change is increasing crop heat stress.
- Molecular mechanisms of heat stress in crops are not well understood.
- Maize (Zea mays) is a vital crop susceptible to heat stress.
Purpose of the Study:
- To investigate the molecular effects of heat stress on maize development.
- To generate a genome-wide transcriptomic atlas of heat stress responses in maize.
- To identify potential regulators for enhancing maize thermotolerance.
Main Methods:
- Generated spatiotemporal mRNA and small RNA transcriptome data across seven maize tissues and three time points.
- Analyzed small RNA profiles, including plastid-derived small RNAs and 24-nt small interfering RNAs from transposable elements.
- Identified heat-responsive microRNA and passenger microRNA species.
Main Results:
- Heat stress induced plastid transcript turnover and repressed photosynthesis-related genes in chloroplasts.
- Reduced abundance of 24-nt small interfering RNAs from transposable elements was observed in maize tassels and roots.
- Identified novel heat-responsive microRNA and passenger microRNA species in maize.
Conclusions:
- Heat stress significantly impacts maize gene expression and small RNA profiles.
- Specific small RNAs and their targets are identified as potential regulators of thermotolerance.
- This study provides a foundation for improving heat tolerance in maize through genetic strategies.
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