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Long non-coding RNA DSCAM-AS1 accelerates the progression of hepatocellular carcinoma via sponging miR-338-3p

Degang Ji1, Guangrui Hu2, Xuanhe Zhang3

  • 1Department of Hepatobiliary Pancreatic Surgery, China-Japan Union Hospital of Jilin University Changchun 130033, Jilin, China.

American Journal of Translational Research
|August 10, 2019
PubMed

Insights

Long non-coding RNA DSCAM-AS1 (Down Syndrome Cell Adhesion Molecule antisense) promotes hepatocellular carcinoma (HCC) progression. This oncogenic lncRNA acts as a sponge for miR-338-3p, driving tumor growth and poor prognosis in HCC patients.

Area of Science:

  • * Molecular Oncology
  • * Cancer Biology
  • * RNA Biology

Background:

  • * Aberrant expression of long non-coding RNA DSCAM-AS1 (Down Syndrome Cell Adhesion Molecule antisense) is implicated in various cancers.
  • * The specific role and regulatory mechanisms of DSCAM-AS1 in hepatocellular carcinoma (HCC) remain largely unelucidated.

Purpose of the Study:

  • * To investigate the expression status, biological functions, and underlying molecular mechanisms of DSCAM-AS1 in HCC.
  • * To determine the potential of DSCAM-AS1 as a diagnostic biomarker and therapeutic target in HCC.

Main Methods:

  • * Quantitative real-time PCR to detect DSCAM-AS1 expression in HCC tissues and serum.
  • * In vitro assays (MTS, wound healing, Transwell) to assess proliferation, migration, and invasion of HCC cells.
  • * Luciferase reporter and RNA immunoprecipitation assays to validate miRNA targeting.
  • * In vivo xenograft mouse models to evaluate the effect of DSCAM-AS1 on tumor growth.

Main Results:

  • * DSCAM-AS1 was significantly upregulated in HCC tissues and serum of HCC patients compared to controls, correlating with poor prognosis.
  • * Knockdown of DSCAM-AS1 inhibited HCC cell proliferation, migration, and invasion.
  • * DSCAM-AS1 directly targets and sponges miR-338-3p, influencing the expression of its targets CyclinD1 and SMO.
  • * Depletion of DSCAM-AS1 significantly suppressed tumor growth in vivo.

Conclusions:

  • * DSCAM-AS1 acts as an oncogenic long non-coding RNA promoting HCC progression.
  • * The DSCAM-AS1/miR-338-3p axis is a key regulatory pathway in HCC development.
  • * DSCAM-AS1 represents a potential diagnostic biomarker and therapeutic target for hepatocellular carcinoma.
Keywords:
DSCAM-AS1Hepatocellular carcinomainvasionmiR-338-3pproliferation

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