Related Experiment Video

Updated: Jan 21, 2026

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing
10:44

In Vitro Selection of Engineered Transcriptional Repressors for Targeted Epigenetic Silencing

Published on: May 5, 2023

1.9K

LncRNA FOXC2 antisense transcript accelerates non-small-cell lung cancer tumorigenesis via silencing p15

Zhe Sun1, Chaozhu He2, Miao Xiao2

  • 1Department of Oncology, The First Affiliated Hospital of Nanchang University Nanchang 330006, China.

American Journal of Translational Research
|August 10, 2019
PubMed

Insights

Long non-coding RNA FOXC2-AS1 promotes non-small cell lung cancer (NSCLC) by repressing p15 expression. Targeting FOXC2-AS1 offers a potential therapeutic strategy for NSCLC patients.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Non-coding RNAs (ncRNAs), particularly long non-coding RNAs (lncRNAs), play a role in cancer development.
  • The specific function of lncRNA FOXC2-AS1 in non-small cell lung cancer (NSCLC) remains largely unknown.

Purpose of the Study:

  • To investigate the role and mechanism of lncRNA FOXC2-AS1 in NSCLC oncogenesis.
  • To explore the potential of FOXC2-AS1 as a therapeutic target for NSCLC.

Main Methods:

  • Correlation analysis between FOXC2-AS1 expression levels and NSCLC patient survival.
  • Functional experiments involving knockdown of FOXC2-AS1 in NSCLC cell lines (A549, H460).
  • Mechanistic studies to elucidate the interaction between FOXC2-AS1, p15, and polycomb repressive complex 2 (PRC2).

Main Results:

  • High expression of FOXC2-AS1 correlates with poor survival in NSCLC patients.
  • Knockdown of FOXC2-AS1 inhibited NSCLC cell proliferation, induced apoptosis, and caused G0/G1 cell cycle arrest.
  • FOXC2-AS1 represses p15 expression by recruiting PRC2 to the p15 promoter, a mechanism confirmed by rescue experiments.

Conclusions:

  • FOXC2-AS1 promotes NSCLC oncogenesis by epigenetically silencing the tumor suppressor gene p15 through PRC2 recruitment.
  • FOXC2-AS1 represents a potential therapeutic target for NSCLC treatment.
Keywords:
EZH2FOXC2-AS1NSCLCPRC2p15

More Related Videos

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells09:16

Investigation of the Transcriptional Role of a RUNX1 Intronic Silencer by CRISPR/Cas9 Ribonucleoprotein in Acute Myeloid Leukemia Cells

Published on: September 1, 2019

8.0K
Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines09:45

Radiosensitivity of Cancer Stem Cells in Lung Cancer Cell Lines

Published on: August 21, 2019

7.5K

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
9.8K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.5K
Transcription01:10

Transcription

Overview
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
155.5K
Transcription Factors02:16

Transcription Factors

Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.3K
Accelerators01:17

Accelerators

Accelerators in concrete serve as admixtures to speed up the hardening process, enabling the concrete to achieve early strength faster. Although accelerators do not necessarily impact the time it takes concrete to set, they reduce this time in practice. A common accelerator is calcium chloride, which is particularly useful for hastening early strength development in cold weather or for rapid repair jobs that require quick heat generation after mixing.
The effectiveness of calcium chloride can...
277
Master Transcription Regulators02:23

Master Transcription Regulators

Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.7K