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Updated: Jan 21, 2026

Low-Cost Cryo-Light Microscopy Stage Fabrication for Correlated Light/Electron Microscopy
Published on: June 5, 2011
Advances in Cryo-Correlative Light and Electron Microscopy: Applications for Studying Molecular and Cellular Events
Sangmi Jun1,2,3, Hyun-Joo Ro4,5,6, Anahita Bharda7
1Drug & Disease Target Team, Korea Basic Science Institute, 162, Yeongudanji-ro, Ochang-eup, Cheongwon-gu, Cheongju-Si, Chungcheongbuk-do, 28119, South Korea. smjun@kbsi.re.kr.
Abstract:
Cryo-correlative light and electron microscopy (Cryo-CLEM) is materializing as a widespread approach amalgamating the advantages of both fluorescence light microscopy (FLM) as well as three dimensional (3D) cryo-electron tomography (cryo-ET) to reveal the ultrastructure of significant target molecules with specific cellular functions. Cryo-CLEM allows imaging of cells by means of fluorescence microscopy exhibiting the location of the destined molecule at high temporal and spatial resolution while cryo-ET is employed to analyze the 3D structure at a molecular resolution in close-to-physiological condition. Present review focuses upon the practical strategies for Cryo-CLEM and recent technical developments that will assist the broad implementation of this technique to investigate and answer questions pertaining to various biological events occurring in the cell.
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