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Related Concept Videos

Flow Cytometry01:23

Flow Cytometry

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The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Identifying Statistically Significant Differences: The F-Test01:14

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The F-test is used to compare two sample variances to each other or compare the sample variance to the population variance. It is used to decide whether an indeterminate error can explain the difference in their values. The underlying assumptions that allow the use of the F-test include the data set or sets are normally distributed, and the data sets are independent of each other. The test statistic F is calculated by dividing one variance by another. In other words, the square of one standard...
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Gene Flow02:39

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Gene flow is the transfer of genes among populations, resulting from either the dispersal of gametes or from the migration of individuals.
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Blood Flow01:29

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Blood is pumped by the heart into the aorta, the largest artery in the body, and then into increasingly smaller arteries, arterioles, and capillaries. The velocity of blood flow decreases with increased cross-sectional blood vessel area. As blood returns to the heart through venules and veins, its velocity increases. The movement of blood is encouraged by smooth muscle in the vessel walls, the movement of skeletal muscle surrounding the vessels, and one-way valves that prevent backflow.
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Couette Flow01:22

Couette Flow

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Couette flow represents the flow of fluid between two parallel plates, with one plate fixed and the other moving with a constant velocity. This configuration allows for a simplified analysis using the Navier-Stokes equations, which govern fluid motion under conditions of viscosity and incompressibility. For Couette flow, the assumptions include a steady, laminar, incompressible flow with a zero-pressure gradient in the flow direction. This flow type is beneficial for understanding shear-driven...
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Laminar Flow01:27

Laminar Flow

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Laminar flow represents a smooth, orderly fluid motion where particles move along parallel paths, resulting in minimal mixing between layers. Streamlined particle paths characterize this flow regime and occur under conditions where viscous forces dominate over inertial forces. The distinction between laminar, transitional, and turbulent flow is primarily determined by the Reynolds number, a dimensionless quantity calculated as:
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Related Experiment Video

Updated: Jan 21, 2026

Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis
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Isolation of Mouse Kidney-Resident CD8+ T cells for Flow Cytometry Analysis

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Flow Cytometry Analysis to Identify Human CD8+ T Cells.

Jacqueline Flynn1,2,3, Paul Gorry4

  • 1Rheumatology Research Group, Centre for Inflammatory Diseases, School of Clinical Sciences at Monash Health, Monash University, Melbourne, VIC, Australia. jacqueline.flynn@monash.edu.

Methods in Molecular Biology (Clifton, N.J.)
|August 10, 2019
PubMed
Summary
This summary is machine-generated.

Flow cytometry is a powerful cell analysis technique. This method uses fluidics, optics, and electronics to detect cell properties and identify cell populations using fluorescently labeled antibodies, specifically for human CD8+ T cell activation status.

Keywords:
ActivationCD markersCD8+ T cellsFlow cytometryMemory T cellsNaïve T cellsT cells

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Flow Cytometry Purification of Mouse Meiotic Cells
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Area of Science:

  • Immunology
  • Cell Biology
  • Biotechnology

Background:

  • Flow cytometry is a versatile technique for multiparameter analysis of single cells.
  • It quantifies physical properties (size, granularity) and chemical characteristics (DNA, mRNA, proteins, receptors).
  • The instrument comprises fluidics, optics, and electronics systems for sample processing and data acquisition.

Purpose of the Study:

  • To outline a protocol for flow cytometry staining and analysis.
  • To specifically detect human CD8+ T cells.
  • To define the activation status of these cells via surface marker phenotyping.

Main Methods:

  • Preparation of a single-cell suspension for antibody staining.
  • Utilizing fluorescently labeled antibodies targeting specific surface markers.
  • Analysis using a flow cytometer with lasers, optics, and detectors.

Main Results:

  • Identification of distinct cell populations based on marker expression.
  • Quantification of cell surface markers to determine activation status.
  • Demonstration of protocol applicability to various cell types by altering antibody targets.

Conclusions:

  • Flow cytometry is crucial for defining cell populations and activation states.
  • Proper antibody selection, titration, and compensation for spectral overlap are essential.
  • This protocol facilitates the specific analysis of human CD8+ T cell activation.