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Updated: Jan 21, 2026

Studying Interactions between Myeloid Cells and CAR T Cells In Vitro and In Vivo
Published on: July 25, 2025
In Vitro Differentiation of T Cell: From CAR-Modified T-iPSC
1Shin Kaneko Laboratory, Department of Cell Growth and Differentiation, Center for iPS Research and Application, Kyoto University, Kyoto, Japan.
Abstract:
T cells engineered to express chimeric antigen receptor (CAR) against the B cell antigen CD19 are achieving remarkable clinical effects on hematological malignancies. Allogeneic transplantation approach is promising for broaden application of CART therapy. iPSCs are one of the ideal cell sources for this approach. CAR-engineered iPSCs are demonstrated to give rise to CAR-engineered T cell and exert their effector function. In this section, we describe the method to generate CAR-engineered iPSCs and differentiate them into T cells.
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