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Related Experiment Videos

Microtitre plate method for alanine transferase (ALT) assays.

A C Archer1, D F Green, E J Buckland

  • 1South West Regional Blood Transfusion Centre, Southmead, Bristol.

Journal of Clinical Pathology
|August 1, 1988
PubMed
Summary

A new kinetic assay for alanine transferase (ALT) offers rapid, cost-effective analysis using microtitre plates. This method provides satisfactory accuracy for ALT levels below 120 IU/l.

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Area of Science:

  • Biochemistry
  • Clinical Chemistry

Background:

  • Alanine transferase (ALT) is a key enzyme marker in liver function diagnostics.
  • Existing ALT assays may have limitations in terms of speed, cost, or automation compatibility.

Purpose of the Study:

  • To develop a novel kinetic assay for alanine transferase (ALT).
  • To leverage microtitre plate technology and commercially available reagents for efficiency.
  • To assess the assay's performance regarding speed, cost, and accuracy.

Main Methods:

  • Development of a kinetic assay for ALT determination.
  • Utilized microtitre plates for high-throughput analysis.
  • Employed commercially available reagents for accessibility and cost-effectiveness.

Main Results:

  • The assay enabled the analysis of 92 samples in under 10 minutes.
  • The cost of apparatus and reagents was found to be competitive with existing methods.
  • Reactions remained linear up to 120 IU/l, with higher concentrations appropriately flagged.
  • Accuracy was satisfactory for ALT concentrations below 120 IU/l.

Conclusions:

  • The developed kinetic ALT assay is efficient and cost-effective.
  • It is suitable for use with multichannel and semiautomated equipment.
  • The assay provides reliable results for clinically relevant ALT concentrations.

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