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Updated: Jan 21, 2026

Preparation and In Vivo Use of an Activity-based Probe for N-acylethanolamine Acid Amidase
Published on: November 23, 2016
Synthesis of Branched Triubiquitin Active-Site Directed Probes
Jiaan Liu1, Yanfeng Li1, Kirandeep K Deol1
1Department of Chemistry, University of Massachusetts, Amherst, Massachusetts 01003, United States.
Abstract:
Active-site directed probes are powerful tools for studying the ubiquitin conjugation and deconjugation machinery. Branched ubiquitin chains have emerged as important proteasome-targeting signals for aggregation-prone proteins and cell cycle regulators. By implementing a new synthetic strategy for the electrophilic warhead, we herein report on the generation and reactivity of a series of branched triubiquitin active-site directed probes. These new tools can be used to dissect the molecular basis of branched chain assembly and disassembly.
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