Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Classifying Matter by Composition03:35

Classifying Matter by Composition

89.6K
Matter: Pure Substances and Mixtures
According to its composition, the matter can be classified into two broad categories — pure substances and mixtures. 
A pure substance is a form of matter that has a constant composition throughout with uniform properties. For example, any sample of sucrose has the same composition and same physical properties, such as melting point, color, and sweetness, regardless of the source from which it is isolated. 
A mixture is composed of two or...
89.6K
Predicting Reaction Outcomes02:24

Predicting Reaction Outcomes

10.3K
Kinetics describes the rate and path by which a reaction occurs. In contrast, thermodynamics deals with state functions and describes the properties, behavior, and components of a system. It is not concerned with the path taken by the process and cannot address the rate at which a reaction occurs. Although it does provide information about what can happen during a reaction process, it does not describe the detailed steps of what appears on an atomic or a molecular level. On the other hand,...
10.3K
Cancer Stem Cells and Tumor Maintenance02:40

Cancer Stem Cells and Tumor Maintenance

5.9K
Early diagnosis and treatment can often cure cancer. However, even with treatment, residual cells called cancer stem cells (CSC) might remain, often causing tumor recurrence. These cancer stem cells possess the potential for self-renewal and multi-lineage differentiation and are often responsible for the therapeutic resistance displayed in most cancers.
Cancer stem cells are thought to originate from tissue-specific normal stem cells or progenitor cells. The normal stem cells usually reside in...
5.9K
Diversity of Archaea I01:30

Diversity of Archaea I

559
Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
559
Diversity of Archaea II01:24

Diversity of Archaea II

462
Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
462
Diversity of Protists I01:15

Diversity of Protists I

870
Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
870

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epithelial memory of inflammation limits tissue damage while promoting pancreatic tumorigenesis.

Science (New York, N.Y.)·2021
Same author

GRP78 expression and prognostic significance in patients with pancreatic ductal adenocarcinoma treated with neoadjuvant therapy versus surgery first.

Pancreatology : official journal of the International Association of Pancreatology (IAP) ... [et al.]·2021
Same author

Elucidation of Tumor-Stromal Heterogeneity and the Ligand-Receptor Interactome by Single-Cell Transcriptomics in Real-world Pancreatic Cancer Biopsies.

Clinical cancer research : an official journal of the American Association for Cancer Research·2021
Same author

Early detection of pancreatic cancer: current state and future opportunities.

Current opinion in gastroenterology·2021
Same author

PRMT1-dependent regulation of RNA metabolism and DNA damage response sustains pancreatic ductal adenocarcinoma.

Nature communications·2021
Same author

Longitudinal assessment of lung clearance index to monitor disease progression in children and adults with cystic fibrosis.

Thorax·2021

Related Experiment Video

Updated: Jan 21, 2026

Author Spotlight: Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
07:31

Author Spotlight: Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue

Published on: May 26, 2023

6.1K

Tumor Microbiome Diversity and Composition Influence Pancreatic Cancer Outcomes.

Erick Riquelme1, Yu Zhang2, Liangliang Zhang3

  • 1Department of Clinical Cancer Prevention, The University of Texas MD Anderson Cancer Center, Houston, TX, USA; Center for Integrative Biology, Faculty of Science, Universidad Mayor, Santiago, Chile.

Cell
|August 10, 2019
PubMed
Summary

The tumor microbiome composition significantly impacts pancreatic ductal adenocarcinoma (PDAC) patient survival. Specific microbial signatures predict long-term survival and influence tumor growth and immune response.

Keywords:
CD8PDACantibioticscancer survivorsfecal microbial transplantsimmunoactivationmicrobiotapancreatic cancertumor microbiome

More Related Videos

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer
07:55

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

Published on: January 17, 2025

1.7K
Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans
07:19

Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans

Published on: September 13, 2022

2.7K

Related Experiment Videos

Last Updated: Jan 21, 2026

Author Spotlight: Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue
07:31

Author Spotlight: Establishment of Pancreatic Cancer-Derived Tumor Organoids and Fibroblasts From Fresh Tissue

Published on: May 26, 2023

6.1K
Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer
07:55

Flow Cytometry-Based Isolation and Therapeutic Evaluation of Tumor-Infiltrating Lymphocytes in a Mouse Model of Pancreatic Cancer

Published on: January 17, 2025

1.7K
Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans
07:19

Compost Microcosms as Microbially Diverse, Natural-like Environments for Microbiome Research in Caenorhabditis elegans

Published on: September 13, 2022

2.7K

Area of Science:

  • Oncology
  • Microbiome Research
  • Immunology

Background:

  • Pancreatic ductal adenocarcinoma (PDAC) has a poor prognosis, with most patients surviving less than 5 years.
  • A subset of PDAC patients exhibits long-term survival, suggesting underlying biological factors influencing disease trajectory.

Purpose of the Study:

  • To investigate the role of the intra-tumoral microbiome in influencing long-term survival in PDAC patients.
  • To identify specific microbial signatures associated with long-term survivorship.
  • To explore the impact of microbiome composition on the host immune response and tumor progression.

Main Methods:

  • 16S rRNA gene sequencing was employed to analyze tumor microbiome composition in PDAC patients with short-term survival (STS) and long-term survival (LTS).
  • Human-into-mice fecal microbiota transplantation (FMT) experiments were conducted using donors with different survival outcomes.
  • Tumor growth and immune cell infiltration were assessed in recipient mice.

Main Results:

  • Higher alpha-diversity in the tumor microbiome was observed in LTS patients compared to STS patients.
  • A specific intra-tumoral microbiome signature (Pseudoxanthomonas-Streptomyces-Saccharopolyspora-Bacillus clausii) was identified as highly predictive of long-term survival.
  • Fecal microbiota transplantation modulated the tumor microbiome, affecting tumor growth and immune infiltration in mice.

Conclusions:

  • PDAC microbiome composition is a critical factor influencing patient survival.
  • The tumor microbiome communicates with the gut microbiome, impacting the host immune system and the overall disease course.
  • Targeting the PDAC microbiome holds potential for improving therapeutic strategies and patient outcomes.