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Updated: Jan 21, 2026
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Probing RNA Structure with Dimethyl Sulfate Mutational Profiling with Sequencing In Vitro and in Cells
Published on: December 9, 2022
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RNA imaging by chemical probes.
Fumiaki Tomoike1, Hiroshi Abe2
1Research Center for Materials Science, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8602, Japan.
Advanced Drug Delivery Reviews
|August 10, 2019
Summary
Detecting intracellular RNA in living cells is challenging. This review classifies chemical probes based on their fluorescence mechanisms for sequence-specific RNA detection and imaging.
Area of Science:
- Molecular Biology
- Biochemistry
- Chemical Biology
Background:
- Intracellular RNA detection is crucial for understanding biological processes.
- Detecting RNA in living cells is difficult due to its complexity and low abundance.
- Chemical probes offer a solution for sequence-specific RNA detection.
Purpose of the Study:
- To review chemical probes for intracellular RNA detection.
- To classify these probes based on their fluorogenic mechanisms.
- To provide examples of RNA imaging in living cells.
Main Methods:
- Classification of chemical probes by fluorogenic mechanism.
- Review of probe design principles (DNA or artificial nucleic acid).
- Discussion of hybridization-triggered fluorescence or chemical reactions.
Main Results:
- Probes utilize sequence-specific hybridization to bind target RNA.
- Fluorogenic mechanisms include fluorophore-quencher interactions, environmental changes, and template reactions.
- Successful examples of RNA imaging in living cells are presented.
Conclusions:
- Chemical probes are essential tools for intracellular RNA analysis.
- Understanding probe mechanisms aids in developing advanced RNA detection strategies.
- This review provides a framework for selecting and designing RNA probes for live-cell imaging.
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