Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Decreased Body Temperature01:29

Decreased Body Temperature

1.0K
A decreased body temperature can occur in patients with hypothermia and frostbite. Heat loss with extended cold exposure overpowers the body's ability to create heat, resulting in hypothermia. Core temperature readings help classify hypothermia. Mild hypothermia is temperatures between 32 °C (89.6 °F) and 35°C (95 °F) and is caused by impaired thermoregulation. Moderate hypothermia is temperatures between 28 C (82.4 °F) and 32 °C (89.6 °F) caused by...
1.0K
Decreased pulse rate01:14

Decreased pulse rate

852
Bradycardia is a medical condition in which the heart rate is slower than normal. It occurs when the heart's natural pacemaker, the sinus node, generates slower electrical impulses than the standard rhythm. In adults, bradycardia is diagnosed when the pulse rate falls below 60 beats per minute, indicating a deviation from the normal heart rate range.
There are specific risk factors that can elevate the likelihood of developing bradycardia. Advanced age is a significant factor, with...
852
Fibronectins Connect Cells with ECM01:25

Fibronectins Connect Cells with ECM

3.3K
Fibronectin is an adhesive glycoprotein present in the extracellular matrix of embryogenic and adult tissue. These molecules primarily aid in regulating cell motility and attachment. A fibronectin molecule is composed of two identical polypeptide chains attached to each other by a pair of disulfide bonds at the C-terminal.
Both proteoglycans and collagen are attached to fibronectin proteins, which, in turn, are attached to integrin proteins. These integrin proteins interact with transmembrane...
3.3K
Magnetic Susceptibility and Permeability01:31

Magnetic Susceptibility and Permeability

2.3K
In linear magnetic materials, like paramagnets and diamagnets, magnetization is proportional to the magnetic field intensity. The constant of proportionality, a dimensionless number, is called magnetic susceptibility. The value of the susceptibility depends on the type of material.
When diamagnetic materials are placed under an external magnetic field, the moments opposite to the field are induced. Hence, the susceptibility for diamagnets has a minimal negative value of 10-5–10-6. Since...
2.3K
Biofilms01:29

Biofilms

1.2K
Biofilms are complex communities of microorganisms encased in a self-produced extracellular polysaccharide matrix attached to surfaces. These microbial consortia can include single or multiple species, providing enhanced survival benefits by forming organized, multilayered structures.The formation of biofilms occurs through four key stages: attachment, colonization, development, and dispersal.During attachment, free-swimming planktonic cells adhere to a surface, often facilitated by...
1.2K
Laminins are the Adhesive Proteins of Basal Lamina00:55

Laminins are the Adhesive Proteins of Basal Lamina

3.1K
Laminins are heterotrimeric proteins with high molecular mass found in the extracellular matrix. Each laminin molecule is composed of three chains, viz. alpha, beta, and gamma, coded by five, four, and three paralogous genes, respectively. Laminins are categories based on the compositions of the three chains.
In humans, the five forms of alpha chains are LAMA 1, LAMA 2, LAMA 3, LAMA 4, and LAMA 5. The four forms of beta chains are LAMB 1, LAMB 2, LAMB 3, and LAMB 4. The three forms of gamma...
3.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Evaluation of the relative expression of genes associated with adherence after different hours of co-culture between Streptococcus uberis and MAC-T cells.

Microbes and infection·2021
Same author

Potential factors involved in the early pathogenesis of Streptococcus uberis mastitis: a review.

Folia microbiologica·2021
Same author

Relative expression of genes associated with adhesion to bovine mammary epithelial cells by Streptococcus uberis.

Research in veterinary science·2020
Same author

Genotyping and study of adherence-related genes of Streptococcus uberis isolates from bovine mastitis.

Microbial pathogenesis·2019
Same author

Streptococcus uberis: In vitro biofilm production in response to carbohydrates and skim milk.

Revista Argentina de microbiologia·2017
Same author

Prevalence and antibiotic susceptibility of coagulase-negative Staphylococcus species from bovine subclinical mastitis in dairy herds in the central region of Argentina.

Revista Argentina de microbiologia·2016
Same journal

Eugenol Microemulsions Exhibit Potent Activity Against Drug-Resistant Diabetic Foot Ulcer Pathogens: Toward Nanocomposite-Based Wound Healing Applications.

Microbial pathogenesis·2026
Same journal

Quercetin and Carvacrol Act Synergistically to Inhibit Candida albicans Biofilms In Vitro via Membrane Disruption and Oxidative Stress.

Microbial pathogenesis·2026
Same journal

Assessment of antimicrobial potency, biological function, and molecular docking of the synthetic peptide IL-20 against the major aquaculture pathogen Vibrio anguillarum.

Microbial pathogenesis·2026
Same journal

2H-Chromen-2-one Disrupts the Staphylococcus aureus Biofilm Matrix and Potentiates Moxifloxacin Activity: In Vitro and In Silico Analyses.

Microbial pathogenesis·2026
Same journal

Serological Diagnosis of Canine Leptospirosis Using a Chimeric Protein Based on Bacterial Surface Antigens.

Microbial pathogenesis·2026
Same journal

Reorganization of the cutaneous immune microenvironment in tegumentary Leishmaniasis-HIV coinfection: a multiparametric in situ immunohistochemical analysis.

Microbial pathogenesis·2026
See all related articles

Related Experiment Video

Updated: Jan 21, 2026

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
07:19

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

Published on: October 21, 2010

46.2K

Fibronectin and laminin induce biofilm formation by Streptococcus uberis and decrease its penicillin susceptibility.

Silvana A Dieser1, Aluminé S Fessia2, Anabella R Zanotti1

  • 1Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km 601, X5804ZAB, Río Cuarto, Córdoba, Argentina.

Microbial Pathogenesis
|August 10, 2019
PubMed
Summary
This summary is machine-generated.

This study reveals fibronectin and laminin enhance Streptococcus uberis biofilm formation, increasing penicillin resistance. Biofilm-growing bacteria require higher antibiotic doses for eradication.

Keywords:
BiofilmFibronectinLamininPenicillinStreptococcus uberis

More Related Videos

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
10:05

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

13.3K
Microtiter Dish Biofilm Formation Assay
03:57

Microtiter Dish Biofilm Formation Assay

Published on: January 30, 2011

114.2K

Related Experiment Videos

Last Updated: Jan 21, 2026

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms
07:19

A 96 Well Microtiter Plate-based Method for Monitoring Formation and Antifungal Susceptibility Testing of Candida albicans Biofilms

Published on: October 21, 2010

46.2K
Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification
10:05

Bile Salt-induced Biofilm Formation in Enteric Pathogens: Techniques for Identification and Quantification

Published on: May 6, 2018

13.3K
Microtiter Dish Biofilm Formation Assay
03:57

Microtiter Dish Biofilm Formation Assay

Published on: January 30, 2011

114.2K

Area of Science:

  • Microbiology
  • Bacterial Pathogenesis
  • Antimicrobial Resistance

Background:

  • Streptococcus uberis is a significant mastitis pathogen.
  • Bacterial biofilm formation contributes to antibiotic tolerance.
  • Extracellular matrix proteins like fibronectin and laminin may influence biofilm development.

Purpose of the Study:

  • To investigate the impact of fibronectin and laminin on in vitro biofilm formation by Streptococcus uberis.
  • To assess the susceptibility of planktonic and biofilm-grown Streptococcus uberis to penicillin.
  • To determine if fibronectin or laminin affects penicillin efficacy against Streptococcus uberis biofilms.

Main Methods:

  • Streptococcus uberis isolates were cultured in Todd Hewitt Broth (THB) with and without fibronectin or laminin.
  • Biofilm production was quantified.
  • Penicillin susceptibility was determined by measuring Minimum Inhibitory Concentrations (MICs), Minimum Biofilm Inhibitory Concentrations (MBICs), and Minimum Biofilm Eradication Concentrations (MBECs).

Main Results:

  • Fibronectin and laminin significantly increased moderate and strong biofilm production by Streptococcus uberis.
  • Planktonic Streptococcus uberis were sensitive to penicillin.
  • Minimum Biofilm Eradication Concentrations (MBECs) for penicillin were substantially higher in the presence of fibronectin or laminin compared to controls.
  • Minimum Biofilm Inhibitory Concentrations (MBICs) were similar regardless of protein supplementation.

Conclusions:

  • Fibronectin and laminin promote Streptococcus uberis biofilm formation.
  • Biofilm formation, particularly when stimulated by fibronectin or laminin, leads to decreased susceptibility to penicillin.
  • Increased biofilm production by Streptococcus uberis may explain therapeutic penicillin failures in treating infections.