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Updated: Jan 21, 2026

Cryo-electron Microscopy Specimen Preparation By Means Of a Focused Ion Beam
Published on: July 26, 2014
Cell stimulation by focused electron beam of atmospheric SEM
Wataru Inami1, Daiki Horiba2, Yoshimasa Kawata1
1Shizuoka University, Graduate School of Science and Technology, Hamamatsu, 4328561, Japan; Shizuoka University, Research Institute of Electronics, Hamamatsu, 4328011, Japan.
Abstract:
Cell stimulation has been performed with a focused electron beam. To protect the live cells from the vacuum environment of the electron beam, the beam irradiated the ambient cells via a thin film. In this way, the cells were electrically stimulated with nanometre resolution in a non-contact process. The response of calcium ion concentration in a single HeLa cell after electron beam irradiation was examined. This technique has the potential to stimulate single ion channels, granules, and organelles.
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