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Synthesis of Graphene-Hydroxyapatite Nanocomposites for Potential Use in Bone Tissue Engineering
Published on: July 27, 2022
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Versatile Biomaterial Platform Enriched with Graphene Oxide and Carbon Nanotubes for Multiple Tissue Engineering
Simona-Rebeca Ignat1, Andreea Daniela Lazăr1, Aida Şelaru1
1Department of Biochemistry and Molecular Biology, University of Bucharest, 050095 Bucharest, Romania.
International Journal of Molecular Sciences
|August 11, 2019
Summary
Novel cellulose acetate membranes enriched with graphene oxide (GO) and carbon nanotubes (CNTs) effectively support human adipose-derived stem cells (hASCs) for tissue engineering applications, enhancing both adipogenic and osteogenic differentiation.
Area of Science:
- Biomaterials Science
- Tissue Engineering
- Nanotechnology
Background:
- Carbon-based nanomaterials like graphene oxide (GO) and carbon nanotubes (CNTs) show promise in medical applications due to their biocompatibility and ability to influence cell behavior.
- Cellulose acetate (CA) membranes are being explored as substrates for tissue regeneration.
Purpose of the Study:
- To investigate the potential of novel flexible cellulose acetate (CA) membranes enriched with GO and CNTs as substrates for soft and hard tissue engineering.
- To evaluate the ability of these CA-CNT-GO membranes to support human adipose-derived stem cells (hASCs) adhesion and differentiation into adipogenic and osteogenic lineages.
Main Methods:
- Fabrication of CA membranes with varying percentages of CNT and GO.
- Assessment of hASCs adhesion, proliferation, and cytoskeleton formation on the membranes.
- Evaluation of adipogenic and osteogenic differentiation markers at gene and protein levels.
- Histological staining and Micro-CT analysis to characterize material morphology and cellular responses.
Main Results:
- Micro-CT revealed porous morphologies with interconnected voids; higher GO-CNT content resulted in thicker walls and larger, more uniform pores, enhancing mechanical stability.
- The addition of 1 wt% GO and CNT significantly improved hASCs adhesion and cytoskeleton formation.
- Both adipogenic and osteogenic differentiation of hASCs were enhanced proportionally to the concentration of GO-CNT in the CA membranes.
Conclusions:
- Cellulose acetate-GO-CNT (CA-CNT-GO) biomaterials exhibit versatile properties suitable for cell differentiation platforms.
- These novel biomaterials show significant potential as future implantable materials for tissue engineering applications, particularly for bone and adipose tissue regeneration.
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