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Related Experiment Video

Updated: Jan 21, 2026

Isolation and Culture of Dental Epithelial Stem Cells from the Adult Mouse Incisor
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Transit amplifying cells coordinate mouse incisor mesenchymal stem cell activation.

Jemma Victoria Walker1, Heng Zhuang2,3, Donald Singer1

  • 1Stem Cells & Regenerative Medicine Laboratory, Peninsula Dental School, University of Plymouth, 16 Research Way, Plymouth, PL6 8BU, UK.

Nature Communications
|August 11, 2019
PubMed
Summary

Mesenchymal stem cells (MSCs) near mouse incisors were identified. Notch signaling and Delta-like 1 homolog (Dlk1) regulate MSCs and transit amplifying cells, impacting tooth regeneration.

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Area of Science:

  • Developmental Biology
  • Stem Cell Biology
  • Regenerative Medicine

Background:

  • Mesenchymal stem cells (MSCs) interact with their microenvironment.
  • Limited molecular links exist between MSCs and mesenchymal transit amplifying cells (MTACs).

Purpose of the Study:

  • To investigate the molecular connections between MSCs and MTACs in mouse incisors.
  • To elucidate the role of Notch signaling and Dlk1 in regulating these cell populations and tooth development.

Main Methods:

  • Utilized mouse incisor model.
  • Employed molecular analysis and lineage tracing techniques.
  • Investigated Notch signaling pathway and Dlk1 expression.

Main Results:

  • Discovered a distinct MSC population adjacent to MTACs and epithelial stem cells.
  • Demonstrated Notch signaling's role in MSC differentiation into MTACs and other mesenchymal lineages.
  • Showed MTACs regulate CL-MSCs via Dlk1, balancing cell numbers and differentiation.
  • Revealed Dlk1's context-dependent function in stem cell priming and self-renewal, influenced by epigenetic control.

Conclusions:

  • Identified a novel MSC population and their regulatory mechanisms in incisor development.
  • Highlighted the crucial roles of Notch signaling and Dlk1 in maintaining stem cell homeostasis and lineage commitment.
  • Findings offer potential for accelerating tooth regeneration and wound healing.