Changes in the cecal microbiota of laying hens during heat stress is mainly associated with reduced feed intake

The aim of the present study was to examine the effect of high temperature on the cecal microbiota of laying hens and analyze the correlation between variation in feed intake, body temperature, and the relative abundance of specific bacterial genera. Two hundred and sixteen 28-wk-old Hy-Line laying hens were randomly divided into 3 groups with 6 replicates and raised in 3 controlled climate chambers. Birds were allocated to either a thermoneutral group (TC, 21 ± 1°C, ad libitum), high cyclic temperature group (HT, 29-35°C, ad libitum), or a pair-feeding group (PF, 21 ± 1°C, pair-feeding on the daily feed intake of HT hens) for 4 wk. There was no significant difference in diversity estimators of laying hens between the HT and TC or PF groups (P > 0.05). Principal component analysis (PCoA) based on unweighted UniFrac distance showed that the HT group did not form a distinct cluster that was clearly separated from the PF group, but was separated from the TC group. Similarity analysis (ANOSIM) confirmed that the TC group was significantly different from the HT (R = 0.259, P = 0.020) and the PF (R = 0.348, P = 0.011) groups, but the HT group was similar with the PF group (R = 0.107, P = 0.153). In addition, there was a significant correlation between feed intake and principal coordinate 1 (PC1) in PCoA (R = -0.494, P = 0.037), but no correlation between core temperature and PC1 (R = 0.025, P = 0.923). Linear discriminant analysis identified 15 genera that discriminated between the HT and TC groups (score ≥2). Among these, the feed intake was significantly related with the relative abundance of 10 genera, however, the core temperature was only significantly correlated with one specific genus. In conclusion, a high cyclic temperature of 29-35°C had significant effects on microbial composition and structure of laying hens, which was mainly related with decreased feed intake.