Related Experiment Video
Updated: Jan 21, 2026
10:09
In Ovo and Ex Ovo Methods to Study Avian Inner Ear Development
Published on: June 16, 2022
2.9K
Cyp1B1 expression patterns in the developing chick inner ear
Sheila Cardeña-Núñez1, Luis Ó Sánchez-Guardado2, Matías Hidalgo-Sánchez1
1Department of Cell Biology, School of Science, University of Extremadura, Badajoz, Spain.
Summary
This study identifies CYP1B1 in the developing chick inner ear, revealing a novel, retinaldehyde dehydrogenase-independent pathway for retinoic acid signaling crucial for inner ear development.
Area of Science:
- Developmental Biology
- Molecular Biology
- Genetics
Background:
- Retinoic acid (RA) is vital for organogenesis, regulating cell proliferation and differentiation.
- In inner ear development, RA signaling primarily involves retinaldehyde dehydrogenase (RALDH) enzymes.
- Cytochrome P450 1B1 (CYP1B1) can generate RA and is found at sites of RA activity.
Purpose of the Study:
- To investigate the presence and role of CYP1B1 in the developing chick inner ear.
- To explore CYP1B1 as a potential RA-signaling mechanism independent of RALDH.
Main Methods:
- In situ hybridization was used to analyze Cyp1B1 gene expression patterns.
- Expression analysis was correlated with developmental stages and known signaling domains (e.g., Fgf10).
Main Results:
- CYP1B1 was detected in the developing chick inner ear for the first time.
- Cyp1B1 expression was observed in specific domains, including Fgf10-positive areas.
- Most sensory patches were Cyp1B1 positive by stage HH34, excluding the macula neglecta.
Conclusions:
- CYP1B1 represents a RALDH-independent mechanism for RA signaling in the developing chick inner ear.
- CYP1B1 expression patterns suggest its involvement in specifying the inner ear's axes.
Related Concept Videos
01:16Anatomy of the Ear
11.2K
Auditory sensation, commonly called hearing, involves the transformation of sonic waves into neural impulses facilitated by the structures of the auditory organ. The prominent, flesh-like structure on the side of the head, called the auricle, directs sound waves towards the auditory canal. The auricle is often mislabeled as the pinna, a term more aligned with mobile structures like a feline's external ear. The auditory canal penetrates the cranium via the external auditory meatus of the...
11.2K
01:06Fixed Action Patterns
17.5K
A fixed action pattern (FAP) is a specific, hard-wired sequence of behaviors that occurs in response to an external stimulus, called a sign stimulus. The behavior is “fixed” because it is essentially unchangeable—proceeding similarly across individuals of a species every time it occurs.
17.5K
02:35Chromatin Position Affects Gene Expression
24.6K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.6K
01:26Patterns of Fever
3.8K
Before understanding the types and patterns of fever, it is essential to know its phases.
3.8K
01:42What is Gene Expression?
194.7K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
194.7K
01:29Oxygen Requirements and Growth Patterns
1.2K
Microorganisms exhibit diverse oxygen requirements and growth patterns driven by their metabolic strategies and environmental adaptations. Oxygen, while essential for many organisms, can also be toxic under certain conditions, shaping how microorganisms grow and survive.Oxygen Requirements of MicroorganismsMicroorganisms are classified based on their ability to use or tolerate oxygen:● Obligate aerobes like Mycobacterium tuberculosis need oxygen for energy production, as it serves as the...
1.2K