Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Complexation Equilibria: Factors Influencing Stability of Complexes01:09

Complexation Equilibria: Factors Influencing Stability of Complexes

807
In complexation reactions, metal cations are the electron pair acceptors, and the ligands are the electron pair donors. The stability of the metal complexes depends primarily on the complexing ability of the central metal ion and the nature of the ligands. Generally, the complexing ability of the metal ion depends on the size and charge of the ion. As the metal ion size increases, the stability of the metal complexes decreases, provided that the valency of the metal ion and the ligands remain...
807
Conformity01:20

Conformity

47.9K
Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
47.9K
Weak Base Solutions03:21

Weak Base Solutions

24.8K
Some compounds produce hydroxide ions when dissolved by chemically reacting with water molecules. In all cases, these compounds react only partially and so are classified as weak bases. These types of compounds are also abundant in nature and important commodities in various technologies. For example, global production of the weak base ammonia is typically well over 100 metric tons annually, being widely used as an agricultural fertilizer, a raw material for chemical synthesis of other...
24.8K
Weak Acid Solutions04:02

Weak Acid Solutions

42.3K
Few compounds act as strong acids. A far greater number of compounds behave as weak acids and only partially react with water, leaving a large majority of dissolved molecules in their original form and generating a relatively small amount of hydronium ions. Weak acids are commonly encountered in nature, being the substances partly responsible for the tangy taste of citrus fruits, the stinging sensation of insect bites, and the unpleasant smells associated with body odor. A familiar example of a...
42.3K
Titration of a Weak Acid with a Weak Base01:08

Titration of a Weak Acid with a Weak Base

4.8K
Weak acids and bases do not undergo dissociation completely, and titrations between these two are rarely studied. When such studies are performed, say, for the titration of a weak acid with a weak base, the titration curve plots the change in pH as a function of the volume of base added. Take the titration of acetic acid with ammonia, for instance. During the titration, these two species form ammonium acetate and water, but the pH change is slow and gradual.
As a result, there is no simple...
4.8K
Titration Calculations: Weak Acid - Strong Base03:55

Titration Calculations: Weak Acid - Strong Base

49.1K
Calculating pH for Titration Solutions: Weak Acid/Strong Base
For the titration of 25.00 mL of 0.100 M CH3CO2H with 0.100 M NaOH, the reaction can be represented as:
49.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

High-throughput protein target mapping enables accelerated bioactivity discovery for ToxCast and PFAS compounds.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Large-scale discovery, analysis and design of protein energy landscapes.

Nature·2026
Same author

Comprehensive mass spectrometry screening-derived atlas of HDAC inhibitors reveals histone-specific acetylation changes.

Cell reports·2026
Same author

Structural basis of NSD2 degradation via targeted recruitment of SCF-FBXO22.

Nature communications·2026
Same author

Identification of E3 ligase substrates and PROTAC-induced ubiquitylation sites using proximity-based identification of ubiquitin sites (PrIUS).

Communications biology·2026
Same author

Dual-site phosphorylation of SLX4 stabilizes the SLX4-MUS81 interface to promote mitotic SMX assembly and genome protection.

Nucleic acids research·2026

Related Experiment Video

Updated: Jan 21, 2026

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K

The MLL1 trimeric catalytic complex is a dynamic conformational ensemble stabilized by multiple weak interactions.

Lilia Kaustov1,2, Alexander Lemak1, Hong Wu3

  • 1Princess Margaret Cancer Centre and Department of Medical Biophysics, University of Toronto, ON M5G 2M9, Canada.

Nucleic Acids Research
|August 11, 2019
PubMed
Summary

This study reveals how the MLL1 complex assembles and functions. We identified new interactions within the histone methyltransferase complex, showing how its catalytic activity can be disrupted.

More Related Videos

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

40.2K
Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

9.2K

Related Experiment Videos

Last Updated: Jan 21, 2026

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis
08:49

Incorporating Target Protein Structure Flexibility and Dynamics in Computational Drug Discovery Using Ensemble-Based Docking Analysis

Published on: June 20, 2025

1.1K
Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

40.2K
Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation
15:05

Deciphering the Structural Effects of Activating EGFR Somatic Mutations with Molecular Dynamics Simulation

Published on: May 20, 2020

9.2K

Area of Science:

  • Epigenetics
  • Molecular Biology
  • Structural Biology

Background:

  • Histone H3K4 methylation is a key epigenetic mark regulating gene transcription.
  • The Mixed Lineage Leukaemia (MLL) family of enzymes, including MLL1, catalyzes this mark.
  • The precise structural mechanisms governing MLL complex assembly and regulation remain unclear.

Purpose of the Study:

  • To investigate the structural basis of MLL1 complex assembly.
  • To elucidate the mechanism of MLL1 complex biochemical function and regulation.
  • To understand the role of subunit interactions in MLL1 activity.

Main Methods:

  • Hybrid methods approach combining small angle X-ray scattering (SAXS), cross-linking mass spectrometry (XL-MS), and nuclear magnetic resonance (NMR) spectroscopy.
  • Computational modeling to generate a dynamic ensemble model of the MLL1 complex.
  • Biochemical assays to assess the impact of disrupting interaction sites on catalytic function.

Main Results:

  • A dynamic ensemble model for the minimally active MLL1 complex (MLL1, WDR5, RbBP5) was generated.
  • A novel interaction site between the MLL1 SET domain and the RbBP5 WD40 domain was identified.
  • Disruption of specific interaction sites was shown to impair the catalytic activity of the MLL1 complex.

Conclusions:

  • Subunit assembly within the MLL1 complex is mediated by multiple weak interaction sites.
  • The identified MLL1-RbBP5 interaction is crucial for complex integrity and function.
  • Understanding these interactions provides insights into the regulation of histone methylation.