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Identification of Circular RNAs using RNA Sequencing
08:25

Identification of Circular RNAs using RNA Sequencing

Published on: November 14, 2019

12.7K

A Novel Circular RNA Mediates Pyroptosis of Diabetic Cardiomyopathy by Functioning as a Competing Endogenous RNA

Fan Yang1, Anqi Li2, Ying Qin3

  • 1Department of Endocrinology, The Second Affiliated Hospital of Harbin Medical University, Harbin, China.

Molecular Therapy. Nucleic Acids
|August 11, 2019
PubMed

Insights

Diabetic cardiomyopathy involves programmed cell death and inflammation. This study identifies a circular RNA (circRNA), CACR, that exacerbates this condition by regulating the miR-214-3p/caspase-1 pathway, suggesting CACR as a potential therapeutic target.

Area of Science:

  • Cardiovascular Biology
  • Molecular Medicine
  • RNA Biology

Background:

  • Diabetic cardiomyopathy (DCM) is a significant cause of mortality in diabetic patients, characterized by cardiomyocyte death and inflammation.
  • Circular RNAs (circRNAs) are emerging as key regulators in disease, but their specific role in DCM requires further investigation.
  • Previous research indicated pyroptosis activation in high-glucose conditions and identified miR-214-3p's regulation of caspase-1.

Purpose of the Study:

  • To investigate the involvement of circRNA in DCM-related pyroptosis.
  • To elucidate the potential mechanism involving the miR-214-3p/caspase-1 pathway.

Main Methods:

  • Identification and naming of a specific circRNA, hsa_circ_0076631, as caspase-1-associated circRNA (CACR).
  • Assessment of CACR levels in high-glucose-treated cardiomyocytes and serum from diabetic patients.
  • Experimental manipulation of CACR and miR-214-3p levels in cardiomyocytes to evaluate effects on pyroptosis and caspase-1 activation.

Main Results:

  • CACR levels were elevated in high-glucose-treated cardiomyocytes and diabetic patient serum.
  • CACR was found to sponge miR-214-3p, inhibiting its expression.
  • Knockdown of CACR mitigated high-glucose-induced caspase-1 activation and pyroptosis.
  • Silencing miR-214-3p partially reversed the protective effects of CACR knockdown.

Conclusions:

  • CACR plays a role in DCM-related pyroptosis via the CACR/miR-214-3p/caspase-1 pathway.
  • CACR represents a potential novel therapeutic target for managing diabetic cardiomyopathy.

Keywords:
caspase-1circular RNAdiabetic cardiomyopathymiR-214-3ppyroptosis

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