Nitrate effects on perchlorate reduction in a H₂/CO₂-based biofilm

The H₂/CO₂-based membrane biofilm reactor (H₂/CO₂-MBfR) that effectively combines microporous diffusions of H₂ and CO₂ is efficient in removing perchlorate (ClO₄^-). Nitrate (NO₃^-) is a common oxidized contaminant frequently coexists with ClO₄^- in water, with the NO₃^- concentration in most ClO₄^--contaminated waters being several orders of magnitude higher than ClO₄^-. Determining the effect of NO₃^- on ClO₄^- reduction is a critical issue in practice. The ClO₄^- reduction performance, biofilm microbial community and influencing mechanism were investigated under a series of feed NO₃^- loadings in this work. ClO₄^- reduction was slightly promoted when NO₃^--N levels were <10 mg/L and inhibited at higher NO₃^--N levels. Denitrification competed more strongly for H₂ than ClO₄^- reduction, regardless of H₂ availability. A higher NO₃^--N loading was a strong driving force to change the biofilm microbial community. Betaproteobacteria were the dominant bacteria at all stages, and the biofilm reactor was enriched in Methyloversatilis and Zoogloea (31.9-56.5% and 10.6-25.8%, respectively). Changes in the relative amounts of Methyloversatilis and Zoogloea coincided with changes in the ClO₄^- fluxes and removal efficiencies and the relative abundances of nitrogen cycle functional genes. These results suggest that Methyloversatilis and Zoogloea likely follow independent reduction mechanisms for ClO₄^- removal.