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Updated: Jan 21, 2026

Multipronged Phenotyping Approaches to Characterize Sugarcane Root Systems
Published on: August 17, 2022
Low-temperature sodium hydroxide pretreatment for ethanol production from sugarcane bagasse without washing process
Qingfeng Wang1, Wen Wang2, Xuesong Tan2
1Guangzhou Institute of Energy Conversion, Chinese Academy of Sciences, CAS Key Laboratory of Renewable Energy, Guangdong Provincial Key Laboratory of New and Renewable Energy Research and Development, Guangzhou 510640, PR China; University of Chinese Academy of Sciences, Beijing 100049, PR China.
Abstract:
A low-temperature sodium hydroxide (NaOH) pretreatment for sugarcane bagasse (SCB) was obtained via the surface response design in this study. However, a large quantity of water consumption and wastewater generation which have been the common problems for alkaline pretreatment of lignocellulose still exists in this pretreatment. In order to reduce water consumption and wastewater generation, this study attempted to perform enzymatic hydrolysis and fermentation of NaOH-treated SCB without washing process. It showed that after pretreatment and solid-liquid separation, NaOH-treated SCB could be directly hydrolysed by cellulase via pH and solid-liquid adjustment without washing steps, and the maximum enzymatic hydrolysis efficiency could reach to 70.2%. A domesticated Saccharomyces cerevisiae Y2034 which can endure 6-times diluted BL was obtained, and realized 67.5% ethanol yield from the enzymatic hydrolysate of unwashed NaOH-treated SCB. It provided a clue for converting NaOH-treated lignocellulose to ethanol at low water consumption and wastewater generation.
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