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High-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast
Published on: April 20, 2017
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Network dynamics of the yeast methyltransferome
Guri Giaever1, Elena Lissina1, Corey Nislow1
1Department of Pharmaceutical Sciences, University of British Columbia, Vancouver, BC, Canada V6T 1Z3.
Microbial Cell (Graz, Austria)
|August 13, 2019
Summary
This study reveals how methyltransferases (MTases) function under stress by analyzing genetic interactions. A key finding links phospholipid methylation to histone methylation, impacting cellular processes.
Area of Science:
- Biochemistry
- Genetics
- Cellular Biology
Background:
- Sulfur assimilation and biosynthesis of methionine, cysteine, and S-adenosylmethionine (SAM) are essential biological processes.
- SAM is a crucial cofactor for methyltransferases (MTases), impacting numerous cellular functions.
- Determining MTase function is challenging due to redundancy, multiple roles, and condition-dependent activities.
Purpose of the Study:
- To systematically analyze the methyltransferome's complexity and plasticity under normal and stress conditions.
- To identify functional relationships and genetic crosstalk between MTases.
- To uncover novel roles for MTases, particularly in relation to cellular stress responses.
Main Methods:
- Performed a comprehensive synthetic genetic analysis of all pairwise MTase double mutations.
- Investigated MTase activity under normal and stress conditions (16°C, 37°C, LiCl).
- Conducted biochemical analyses of the histone H3K4 COMPASS complex and OPI3.
Main Results:
- Generated a network of MTase interactions revealing the methyltransferome's complexity and plasticity.
- Identified condition-dependent functional relationships among MTases.
- Discovered a novel role for a phospholipid methyltransferase in histone H3K4 methylation, linking lipid homeostasis to epigenetic regulation.
Conclusions:
- The study provides a valuable resource for understanding MTase function and methyltransferome dynamics.
- Findings highlight the interplay between lipid metabolism and histone methylation.
- The research offers insights into genetic crosstalk and cellular responses to stress.
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