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Updated: Jan 21, 2026

Ultrasonic-augmented Primary Adult Fibroblast Isolation
Published on: July 29, 2019
Ultrasonic-augmented Primary Adult Fibroblast Isolation
Stephan R Künzel1, Charlotte Schaeffer2, Karolina Sekeres2
1Institute of Pharmacology and Toxicology, Faculty of Medicine Carl Gustav Carus, Technische Universität Dresden; Stephan.Kuenzel@tu-dresden.de.
Abstract:
Primary adult fibroblasts have become an important tool to study fibrosis, fibroblast interactions and inflammation in all body tissues. Since primary fibroblasts cannot divide indefinitely due to myofibroblast differentiation or senescence induction, new cultures must be established regularly. However, there are several obstacles to overcome during the processes of developing a reliable isolation protocol and primary fibroblast isolation itself: the method's degree of difficulty (especially for beginners), the risk of bacterial contamination, the required time until primary fibroblasts can be used for experiments, and subsequent cell quality and viability. In this study, a fast, reliable and easy-to-learn protocol to isolate and culture primary adult fibroblasts from mouse heart, lung, liver and kidney combining enzymatic digestion and ultrasonic agitation is provided.
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