Artificial Metalloenzymes: Challenges and Opportunities
Holly J Davis1, Thomas R Ward1
1University of Basel, BPR 1096, Mattenstrasse 24a, CH-4058 Basel, Switzerland.
ACS Central Science
|August 13, 2019
Summary
Artificial metalloenzymes (ArMs) combine metal cofactors with protein scaffolds for novel catalysis. Recent advancements showcase diverse assembly methods and scaffold designs, addressing key challenges in the field.
Area of Science:
- Biochemistry
- Catalysis
- Protein Engineering
Background:
- Artificial metalloenzymes (ArMs) integrate abiotic metal cofactors into protein scaffolds.
- The field has evolved significantly over 60 years, expanding reaction classes and assembly strategies.
Purpose of the Study:
- To review prominent recent examples of artificial metalloenzymes.
- To highlight current challenges and future opportunities in ArM development.
Main Methods:
- Discussing various ArM assembly techniques, including covalent and supramolecular anchoring.
- Examining diverse protein scaffold selection and engineering approaches (evolution, repurposing, in silico design).
Main Results:
- Showcasing the versatility of ArMs across different reaction types.
- Illustrating successful integration of metal cofactors into protein scaffolds.
Conclusions:
- ArM development presents significant challenges but offers vast opportunities for innovative catalysis.
- Continued research into assembly and scaffold design is crucial for advancing the field.
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