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Infection of established cell lines with Campylobacter mucosalis
M Rajasekhar1, R E Okereke, G H Lawson
1Department of Veterinary Pathology, University of Edinburgh, Roslin, Midlothian, Gt. Britain.
Abstract:
Campylobacter mucosalis (CM) and other Campylobacter spp. mainly from cases of the porcine proliferative enteropathies were used to infect cell line cultures. CM attached to and persisted intracellularly in PK (15), MDBK, BHK, HeLa, INT-407 and other cells; in PK (15) this infection resulted in a marked cytopathic effect and total destruction of the cell culture in 10 days. MDBK, INT-407, BHK and HeLa cultures were more resistant to the effects of infection. CM did not adhere to Vero, LLCMK2 or CEF cells and no cytopathic changes took place. The other Campylobacter spp tested did not attach to cell cultures and cell death occurred within 4 days. Attempts to passage CM were unsuccessful due to a decrease in the number of bacteria at each passage.
Insights
Campylobacter mucosalis (CM) infected various cell lines, causing significant damage to PK (15) cells. Other Campylobacter species did not adhere to cells, indicating CM
Area of Science:
- Veterinary Microbiology
- Cell Biology
- Bacterial Pathogenesis
Background:
- Porcine proliferative enteropathies are significant swine diseases.
- Campylobacter species are implicated in enteric diseases.
- Understanding bacterial-host cell interactions is crucial for disease control.
Purpose of the Study:
- To investigate the interaction of Campylobacter mucosalis (CM) and other Campylobacter spp. with various cell lines.
- To determine the cytopathic effects of CM infection on different cell types.
- To assess the susceptibility of various cell lines to Campylobacter infection.
Main Methods:
- Infection of multiple cell lines (PK (15), MDBK, BHK, HeLa, INT-407, Vero, LLCMK2, CEF) with CM and other Campylobacter spp.
- Observation of bacterial adherence, intracellular persistence, and cytopathic effects.
- Assessment of cell culture destruction and bacterial passaging.
Main Results:
- CM adhered to and persisted intracellularly in PK (15), MDBK, BHK, HeLa, and INT-407 cells.
- PK (15) cell cultures showed marked cytopathic effects and complete destruction within 10 days post-infection with CM.
- Vero, LLCMK2, and CEF cells were resistant to CM adherence and infection; other Campylobacter spp. did not adhere and caused rapid cell death.
Conclusions:
- Campylobacter mucosalis exhibits specific cell tropism, effectively infecting and destroying certain cell lines like PK (15).
- Differential susceptibility of cell lines to CM infection highlights variations in host-pathogen interactions.
- The inability to passage CM suggests challenges in maintaining bacterial viability in vitro, impacting further research.