Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Facilitated Transport01:19

Facilitated Transport

146.3K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
146.3K
Facilitated Transport01:19

Facilitated Transport

18.1K
The chemical and physical properties of plasma membranes cause them to be selectively permeable. Since plasma membranes have both hydrophobic and hydrophilic regions, substances need to be able to transverse both regions. The hydrophobic area of membranes repels substances such as charged ions. Therefore, such substances need special membrane proteins to cross a membrane successfully. In  facilitated transport, also known as facilitated diffusion, molecules and ions travel across a...
18.1K
Automatic Processing and Automatic Social Behavior01:28

Automatic Processing and Automatic Social Behavior

222
Automatic processing refers to the cognitive operations that occur without conscious intent or awareness, playing a fundamental role in shaping social cognition and behavior. These processes enable individuals to navigate complex social environments efficiently by relying on mental shortcuts and pre-existing knowledge structures known as schemas. One of the most influential mechanisms underlying automatic processing is priming, which subtly activates mental representations through exposure to...
222
Social Facilitation01:04

Social Facilitation

36.0K
Not all intergroup interactions lead to negative outcomes. Sometimes, being in a group situation can improve performance. Social facilitation occurs when an individual performs better when an audience is watching than when the individual performs the behavior alone. This typically occurs when people are performing a task for which they are skilled.
36.0K
Facilitated Diffusion01:16

Facilitated Diffusion

1.2K
The plasma membrane, a critical structure in cellular biology, houses an array of transporters, or carrier proteins, interspersed within its lipid bilayer. These proteins play a crucial role in solute transport through facilitated diffusion, a form of passive diffusion that uses transporters to move the molecules across the membrane.
In this process, substrates such as organic compounds and ions interact with a transporter on one side, triggering conformational changes in proteins that enable...
1.2K
Matrix Proteoglycans and Glycoproteins01:21

Matrix Proteoglycans and Glycoproteins

4.9K
Proteoglycans are extensively glycosylated proteins, commonly found in the extracellular matrix, interwoven with collagen fibers. Hyaline cartilage, the most common type of cartilage in the body, consists of short and dispersed collagen fibers associated with large amounts of proteoglycans. These proteoglycans have long negative charges that attract cations, which in turn attract water molecules. This influx of ions and water molecules swells up the proteoglycan like a water-soaked gel that can...
4.9K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Deletion of neurosecretory proteins GL and GM drives dual anti-obesity effects via appetite suppression and enhanced energy expenditure.

Communications biology·2026
Same author

Correction: Sleep-wake patterns are altered with age, Prdm13 signaling in the DMH and diet restriction in mice.

Life science alliance·2026
Same author

A translocation-mediated duplication of the distal region of chromosome 5A reduced the culm length in a gamma-irradiated wheat mutant.

Molecular breeding : new strategies in plant improvement·2026
Same author

Reduced adverse effects of infrared free electron laser-irradiated insulin amyloid in vitro and in mice.

Biochimica et biophysica acta. General subjects·2026
Same author

A hierarchical lectin-based multimodal workflow for spatial mapping of extracellular matrix glycans in fibrotic hearts.

Analytical and bioanalytical chemistry·2026
Same author

Luminescent Donor-Acceptor Radical With Propeller Chirality: Bright and Photostable Red Circularly Polarized Luminescence and Whispering Gallery Mode Resonance.

Angewandte Chemie (International ed. in English)·2026

Related Experiment Video

Updated: Jan 21, 2026

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
09:22

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Published on: October 13, 2017

12.3K

Lectin Bead Array in a Single Tip Facilitates Fully Automatic Glycoprotein Profiling.

Hiroko Shimazaki1, Kozue Saito1, Atsushi Matsuda1

  • 1Glycoscience and Glycotechnology Research Group, Biotechnology Research Institute for Drug Discovery , National Institute of Advanced Industrial Science and Technology , Tsukuba , Ibaraki 305-8568 , Japan.

Analytical Chemistry
|August 14, 2019
PubMed
Summary
This summary is machine-generated.

This study introduces an automated system for analyzing glycan alterations in glycoproteins, crucial for developing new diagnostic tools. The novel "bead arrays in a single tip" method enables precise, low-variation glycan profiling for potential clinical applications.

More Related Videos

Easy and Accurate Mechano-profiling on Micropost Arrays
10:25

Easy and Accurate Mechano-profiling on Micropost Arrays

Published on: November 17, 2015

11.6K
Automatic Laser-based Geometry Capture for Finite Element Analysis of Weld Beads
07:58

Automatic Laser-based Geometry Capture for Finite Element Analysis of Weld Beads

Published on: July 25, 2025

775

Related Experiment Videos

Last Updated: Jan 21, 2026

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles
09:22

Bead Based Multiplex Assay for Analysis of Tear Cytokine Profiles

Published on: October 13, 2017

12.3K
Easy and Accurate Mechano-profiling on Micropost Arrays
10:25

Easy and Accurate Mechano-profiling on Micropost Arrays

Published on: November 17, 2015

11.6K
Automatic Laser-based Geometry Capture for Finite Element Analysis of Weld Beads
07:58

Automatic Laser-based Geometry Capture for Finite Element Analysis of Weld Beads

Published on: July 25, 2025

775

Area of Science:

  • Biochemistry
  • Analytical Chemistry
  • Glycomics

Background:

  • Glyco-alteration in diseases necessitates quantitative diagnostic agents.
  • Omics technologies have advanced analytical systems for disease monitoring.
  • Clinical implementation requires simple, automated analytical apparatus.

Purpose of the Study:

  • To develop an automated system for quantitative characterization of glyco-alteration.
  • To enable precise glycan profiling from minimal glycoprotein samples.
  • To facilitate clinical use of lectin arrays for disease monitoring.

Main Methods:

  • Utilized a unique "bead arrays in a single tip" strategy for glycan analysis.
  • Developed an alternative lectin array for minimal characterization of glycan profiles.
  • Employed a simple autopipetting robot for chemiluminescence detection of glycan-lectin interactions.

Main Results:

  • Achieved precise chemiluminescence detection with a wide dynamic range, surpassing fluorescence-based arrays.
  • Demonstrated automatic glycan profiling from sample pretreatment to detection.
  • Obtained low variation and linear detection using the tip-based array format.

Conclusions:

  • The developed automatic analysis system offers a robust method for quantitative glycan profiling.
  • The system's efficiency and precision may facilitate its adoption in clinical practice.
  • This lectin array technology holds promise for in vitro diagnostic agent development.