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Published on: July 22, 2020
Assessment of MMP29 Gene Expression and Silver Nanoparticles Effects on Colon Cancer Cell Line (HT29)
Alaa Naseer Mohammed Ali1, Sawsan Mohammed Kareem2, Abdolmajid Ghasemian3
1Department of Biology, College of Science, Mustansiryiah University, Baghdad, Iraq.
Background:
Colon cancer is a major cause of death around the world. The evaluation of novel approaches on corresponding genes would be a vital strategy toward eradication of cancer cells. In this study, the toxicity of silver nanoparticle on the colon cancer cell line (HT29) and expression of matrix metalloproteinase (MMP29) gene was investigated.
Materials And Methods:
The silver nanoparticle (AgNPs) was synthesized and assessed by transmission electron microscopy (TEM). The cytotoxicity of synthesized AgNP on the HT29 cell line was evaluated using the MTT assay. Furthermore, the expression of MMP29 gene was investigated by the quantitative real-time PCR (RT-qPCR).
Results:
The TEM results revealed that the fabricated AgNPs were mostly spherical in shape and had an average diameter of 22 nm. The results outlined that AgNPs significantly decreased the viability of cells in a dose- and time-dependent manner (p < 0.001). Additionally, we observed a significant difference among various concentrations.
Conclusion:
The findings indicated that the green fabricated AgNPs have the potential as a promising approach toward the colon cancer therapy. Furthered studies are essential to evaluate against other cancer cell lines and genes participating in the cancer progress.
Insights
Green synthesized silver nanoparticles (AgNPs) show promise in colon cancer therapy by reducing HT29 cell viability. Further research is needed to explore their potential against other cancer types and genes.
Area of Science:
- Nanotechnology
- Oncology
- Molecular Biology
Background:
- Colon cancer remains a significant global health challenge.
- Investigating novel therapeutic strategies targeting cancer genes is crucial.
- This study explores silver nanoparticles' effects on colon cancer.
Purpose of the Study:
- To synthesize and characterize silver nanoparticles (AgNPs).
- To evaluate the cytotoxicity of AgNPs on the HT29 colon cancer cell line.
- To investigate the effect of AgNPs on matrix metalloproteinase 29 (MMP29) gene expression.
Main Methods:
- AgNPs synthesized and characterized using transmission electron microscopy (TEM).
- Cytotoxicity assessed via MTT assay on HT29 cells.
- MMP29 gene expression analyzed using quantitative real-time PCR (RT-qPCR).
Main Results:
- TEM confirmed spherical AgNPs with an average diameter of 22 nm.
- AgNPs significantly reduced HT29 cell viability in a dose- and time-dependent manner (p < 0.001).
- Significant differences in cell viability were observed across various AgNP concentrations.
Conclusions:
- Green synthesized AgNPs demonstrate potential as a therapeutic approach for colon cancer.
- Further studies are warranted to evaluate AgNPs against other cancer cell lines and genes involved in cancer progression.
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