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Updated: Jan 21, 2026

Transmembrane Domain Oligomerization Propensity determined by ToxR Assay
Published on: May 26, 2011
Colocalization Strategy Unveils an Underside Binding Site in the Transmembrane Domain of Smoothened Receptor
Fang Zhou1,2,3,4, Kang Ding1,5,3,4, Yiqing Zhou6,7
1iHuman Institute , ShanghaiTech University , Ren Building, 393 Middle Huaxia Road , Pudong New District, Shanghai 201210 , China.
Abstract:
We unveiled an underside binding site on smoothened receptor (SMO) by a colocalization strategy using two structurally complementary photoaffinity probes derived from a known ligand Allo-1. Docking study and structural dissection identified key interactions within the site, including hydrogen bonding, π-π interactions, and hydrophobic interactions between Allo-1 and its contacting residues. Taken together, our results reveal the molecular base of Allo-1 binding and provide a basis for the design of new-generation ligands to overcome drug resistance.
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