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Updated: Jan 21, 2026

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CD82 controls CpG-dependent TLR9 signaling

Nida S Khan1,2,3,4, Daniel P Lukason1, Marianela Feliu1

  • 1Division of Infectious Disease, Department of Medicine, Massachusetts General Hospital, Boston, Massachusetts, USA.

FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology
|August 14, 2019
PubMed

Insights

The tetraspanin CD82 regulates Toll-like receptor 9 (TLR9) signaling, crucial for innate immunity. This study reveals CD82

Area of Science:

  • Immunology
  • Cell Biology
  • Molecular Biology

Background:

  • The tetraspanin CD82 is known to suppress tumor metastasis and regulate cell processes.
  • Its role in innate immunity, particularly in Toll-like receptor (TLR) signaling, remains unexplored.

Purpose of the Study:

  • To investigate the function of CD82 in the innate immune response.
  • To elucidate the mechanisms by which CD82 influences TLR9 trafficking and signaling.

Main Methods:

  • Co-immunoprecipitation assays to detect TLR9 and CD82 association in macrophages.
  • Confocal microscopy to visualize TLR9 and CD82 localization.
  • Analysis of myddosome formation and NF-κB nuclear translocation in response to CpG stimulation.

Main Results:

  • CD82 and TLR9 were found to associate in macrophages within the endoplasmic reticulum and post-ER compartments.
  • CD82 is essential for the formation of the myddosome complex upon CpG stimulation.
  • CD82 modulates TLR9-dependent NF-κB activation, impacting inflammatory cytokine production.

Conclusions:

  • CD82 is a key regulator of TLR9 trafficking and signaling pathways.
  • This finding represents the first implication of a tetraspanin in TLR signaling regulation.
  • CD82's role in TLR9 signaling has potential implications for cancer immunotherapy and host defense against pathogens.
Keywords:
TLRsmacrophagesmyddosometetraspanins

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