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A Step by Step Protocol for Subretinal Surgery in Rabbits
Published on: September 13, 2016
Monosynaptic tracing: a step-by-step protocol
Thomas K Lavin1, Lei Jin1, Ian R Wickersham1
1McGovern Institute for Brain Research, Massachusetts Institute of Technology, Cambridge, MA, United States.
Abstract:
Monosynaptic tracing using deletion-mutant rabies virus allows whole-brain mapping of neurons that are directly presynaptic to a targeted population of neurons. The most common and robust way of implementing it is to use Cre mouse lines in combination with Cre-dependent adeno-associated viral vectors for expression of the required genes in the targeted neurons before subsequent injection of the rabies virus. Here we present a step-by-step protocol for performing such experiments using first-generation (ΔG) rabies viral vectors.
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