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Related Concept Videos

RNA-seq03:21

RNA-seq

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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
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Adult Stem Cells01:33

Adult Stem Cells

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Stem cells are undifferentiated cells that divide and produce more stem cells or progenitor cells that differentiate into mature, specialized cell types. All the cells in the body are generated from stem cells in the early embryo, but small populations of stem cells are also present in many adult tissues including the bone marrow, brain, skin, and gut. These adult stem cells typically produce the various cell types found in that tissue—to replace cells that are damaged or to continuously...
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Embryonic Stem Cells00:58

Embryonic Stem Cells

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Embryonic stem (ES) cells are undifferentiated pluripotent cells, meaning they can produce any cell type in the body. This gives them tremendous potential in science and medicine since they can generate specific cell types for use in research or to replace body cells lost due to damage or disease.
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Transfer RNA Synthesis02:36

Transfer RNA Synthesis

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One of the unique features of tRNA is the presence of modified bases. In some tRNAs, modified bases account for nearly 20% of the total bases in the molecule. Altogether, these unusual bases protect the tRNA from enzymatic degradation by RNases.
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Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

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Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore...
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RNA Interference01:23

RNA Interference

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RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
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Related Experiment Video

Updated: Jan 21, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
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Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis

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Transcriptomic Profiling of Adipose Derived Stem Cells Undergoing Osteogenesis by RNA-Seq.

Shahensha Shaik1, Elizabeth C Martin2, Daniel J Hayes3

  • 1Bioengineering Laboratory, Department of Mechanical Engineering, Louisiana State University, Baton Rouge, LA, USA.

Scientific Reports
|August 15, 2019
PubMed
Summary

Adipose-derived stromal/stem cells (ASCs) undergoing osteogenesis show significant changes in extracellular matrix organization and angiogenesis pathways. This study reveals key gene expression shifts regulating bone development and vascularization.

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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Last Updated: Jan 21, 2026

Isolation and Enrichment of Human Adipose-derived Stromal Cells for Enhanced Osteogenesis
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues
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Isolation and Differentiation of Adipose-Derived Stem Cells from Porcine Subcutaneous Adipose Tissues

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Area of Science:

  • Stem Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • Adipose-derived stromal/stem cells (ASCs) are multipotent cells capable of differentiating into various lineages, including bone cells.
  • Cell differentiation is a complex process influenced by intracellular pathways, cell-secretome interactions, and extracellular matrix (ECM) signaling.
  • Understanding the genetic underpinnings of ASC osteogenesis is crucial for regenerative medicine and bone tissue engineering.

Purpose of the Study:

  • To identify the transcriptome profile of osteogenically induced ASCs using RNA-sequencing (RNA-Seq).
  • To investigate the expression patterns of genes involved in ECM organization, angiogenesis, and key regulatory pathways during ASC osteogenesis.
  • To validate RNA-Seq findings through quantitative PCR (QPCR) analysis.

Main Methods:

  • RNA-sequencing (RNA-Seq) was employed to profile gene expression in osteogenically induced ASCs.
  • Gene Ontology (GO) functional annotation analysis was performed using DAVID bioinformatics resources.
  • Quantitative PCR (QPCR) was used to validate the expression of selected differentially expressed genes.

Main Results:

  • RNA-Seq analysis revealed significant enrichment of pathways related to ECM organization and angiogenesis.
  • Upregulation of pro-angiogenic chemokines and ECM remodeling enzymes (MMPs, ADAMTSs) was observed during osteogenesis.
  • Key regulatory genes involved in WNT, TGF-β, JNK, Hedgehog, and ERK1/2 pathways showed increased expression, indicating pathway interplay.

Conclusions:

  • Osteogenic differentiation of ASCs involves substantial changes in ECM composition and angiogenic signaling.
  • The secretome of differentiating ASCs may play a role in vascular development and bone integration.
  • Interplay between multiple signaling pathways critically regulates ASC osteogenesis.